GOT1 Protein (AA 2-414) (His tag)

Catalog No. ABIN1096059

Product Details

Target: GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))

Protein Type: Recombinant

Protein Characteristics: AA 2-414

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This GOT1 protein is labelled with His tag.

Purpose: Recombinant Human Glutamate Oxaloacetate Transaminase 1/GOT1 (C-6His)

Sequence: APPSVFAEVP QAQPVLVFKL TADFREDPDP RKVNLGVGAY RTDDCHPWVL PVVKKVEQKI ANDNSLNHEY LPILGLAEFR SCASRLALGD DSPALKEKRV GGVQSLGGTG ALRIGADFLA RWYNGTNNKN TPVYVSSPTW ENHNAVFSAA GFKDIRSYRY WDAEKRGLDL QGFLNDLENA PEFSIVVLHA CAHNPTGIDP TPEQWKQIAS VMKHRFLFPF FDSAYQGFAS GNLERDAWAI RYFVSEGFEF FCAQSFSKNF GLYNERVGNL TVVGKEPESI LQVLSQMEKI VRITWSNPPA QGARIVASTL SNPELFEEWT GNVKTMADRI LTMRSELRAR LEALKTPGTW NHITDQIGMF SFTGLNPKQV EYLVNEKHIY LLPSGRINVS GLTTKNLDYV ATSIHEAVTK IQLEHHHHHH

Characteristics: Recombinant Human Glutamate Oxaloacetate Transaminase 1/GOT1 is produced with our E. coli expression system. The target protein is expressed with sequence (Ala2-Leu414) of Human GOT1.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 100 mM NaCl, 2 mM DTT, 20 % Glycerol, pH 7.5 .

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))

Alternative Name: Glutamate Oxaloacetate Transaminase 1/GOT1 (GOT1 Products)

Synonyms: ASTQTL1 Protein, GIG18 Protein, cAspAT Protein, cCAT Protein, AST Protein, ISSD Protein, NSD Protein, SD Protein, SIALIN Protein, SIASD Protein, SLD Protein, xr406 Protein, CASPAT Protein, ASPARTATE AMINOTRANSFERASE Protein, ATAAT Protein, MATERNAL EFFECT EMBRYO ARREST 17 Protein, MEE17 Protein, T26C19.9 Protein, T26C19_9 Protein, aspartate aminotransferase Protein, aatA Protein, 83.t00021 Protein, AI789014 Protein, Got-1 Protein, Aspat Protein, Gaspat Protein, AL022787 Protein, FABP-pm Protein, Got-2 Protein, mAspAT Protein, ASPATA Protein, glutamic-oxaloacetic transaminase 1 Protein, solute carrier family 17 member 5 Protein, glutamic-oxaloacetic transaminase 1 homeolog Protein, aspartate aminotransferase Protein, glutamic-oxaloacetic transaminase 1, soluble Protein, glutamic-oxaloacetic transaminase 2 Protein, glutamatic-oxaloacetic transaminase 2, mitochondrial Protein, GOT1 Protein, SLC17A5 Protein, got1.S Protein, AAT Protein, aspC Protein, EHI_006080 Protein, AST2 Protein, ast2 Protein, got1 Protein, Got1 Protein, GOT2 Protein, Got2 Protein

Background: Glutamate Oxaloacetate Transaminase 1 (GOT1) is a cytoplasmic protein. GOT1 belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. GOT1 is a pyridoxal phosphate-dependent enzyme that exists in cytoplasmic and mitochondrial forms. GOT1 plays a key role in amino acid metabolism and the urea and tricarboxylic acid cycles. GOT1 involves in L-methionine salvage from methylthioadenosine, aspartate catabolic process, cellular response to insulin stimulus, polyamine metabolic process, and glucocorticoid stimulus.
Alternative Names: Aspartate Aminotransferase Cytoplasmic, Glutamate Oxaloacetate Transaminase 1, Transaminase A, GOT1

Molecular Weight: 47.31 kDa

UniProt: P17174

Pathways: Hepatitis C, Monocarboxylic Acid Catabolic Process, Methionine Biosynthetic Process