FGF2 Protein (AA 135-288, Isoform 1)

Catalog No. ABIN2017855

Product Details

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: Isoform 1, AA 135-288

Origin: Human

Source: Escherichia coli (E. coli)

Characteristics: ED50 < 0.25 ng/mL, measured by the cell proliferation assay using 3T3 cells, corresponding to a specific activity of > 4 x 10^6 units/mg.

Purity: > 95 % by SDS-PAGE analysis.

Endotoxin Level: < 0.2 EU/μg, determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Storage: -80 °C

Storage Comment: Lyophilized recombinant human Fibroblast Growth Factor-basic (rhFGF-basic) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhFGF-basic remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.

Expiry Date: 6 months

Target Details

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Alternative Name: Fibroblast Growth Factor-Basic (FGF-Basic) (FGF2 Products)

Background: Fibroblast Growth Factor-basic (FGF-basic), also known as FGF-2, is a pleiotropic cytokine and one of the prototypic members of the heparin-binding FGF family. Like other FGF family members, bFGF has the beta trefoil structure. In vivo, bFGF is produced by a variety of cells, including cardiomycotes, fibroblasts, and vascular cells. bFGF regulates a variety of processes including cell proliferation, differentiation, survival, adhesion, motility, apoptosis, limb formation and wound healing. bFGF can be tumorigenic due to its role in angiogenesis and blood vessel remodeling. The angiogenic effects of bFGF can produce beneficial cardioprotection during acute heart injury.Recombinant human Fibroblast Growth Factor-basic (rhFGF-basic) produced in E. coli is a single non-glycosylated polypeptide chain containing 154 amino acids. A fully biologically active molecule, rhFGF-basic has a molecular mass of 17.1 kDa analyzed by reducing SDS-PAGE.
Synonyms: Fibroblast Growth Factor-basic, FGF-2, HBGF-2, Prostatropin

Molecular Weight: 17.1 kDa, observed by reducing SDS-PAGE.

UniProt: P09038

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteins