IL6RA Protein (AA 20-365) (His tag)

Catalog No. ABIN2181394

Product Details

Target: IL6RA (Interleukin 6 Receptor, alpha (IL6RA))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 20-365

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This IL6RA protein is labelled with His tag.

Sequence: AA 20-365

Characteristics: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 40 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Purity: >92 % as determined by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).

References

Tanemura, Mochizuki, Kumachi, Nemoto: "Easy and rapid binding assay for functional analysis of disulfide-containing peptides by a pull-down method using a puromycin-linker and a cell-free translation system." in: Biology, Vol. 4, Issue 1, pp. 161-72, (2015) (PubMed).

Target Details

Target: IL6RA (Interleukin 6 Receptor, alpha (IL6RA))

Alternative Name: IL-6 R alpha (IL6RA Products)

Background: Interleukin 6 receptor (IL6R) is also known as CD126 (Cluster of Differentiation 126) , is a potent pleiotropic cytokine that regulates cell growth and differentiation of various tissues, and is known particularly for its role in the immune response and acute phase reactions. IL6R is a protein complex consisting of a IL-6 receptor subunit (IL6R) and interleukin 6 signal transducer Glycoprotein 130. IL6R also denotes the human gene encoding this subunit. Alternatively spliced transcript variants encoding distinct isoforms have been reported. IL6R subunit also shared by many other cytokines. The soluble form of IL6R arises from proteolytic cleavage of membrane-bound IL6Rα, and acts agonistically by making the IL6 ligand accessible to the signal transducer gp130. Dysregulated production of IL6 and IL6R are implicated in the pathogenesis of several inflammatory diseases and malignancies such as multiple myeloma, rheumatoid arthritis, or osteoporosis, and it has been reported that a humanized anti-IL6R monoclonal antibody is a promising agent applicable to the therapeutic approach for IL6 driven diseases. Interleukin-6 receptor has been shown to interact with Interleukin 6 and Ciliary neurotrophic factor.

Molecular Weight: 39.4 kDa

NCBI Accession: NP_000556

Pathways: JAK-STAT Signaling, Autophagy, Growth Factor Binding, Cancer Immune Checkpoints