ATG7 Protein (AA 450-698) (His tag)

Catalog No. ABIN2468252

Product Details

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Protein Type: Recombinant

Protein Characteristics: AA 450-698

Origin: Mouse

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This ATG7 protein is labelled with His tag.

Application: Western Blotting (WB), ELISA, Mass Spectrometry (MS), Neutralization (Neut)

Purity: ~95 %

Application Details

Application Notes: This recombinant protein can be used for WB, ELISA, MS and neutralization assays.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 1X PBS containing 10 % glycerol

Handling Advice: Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Storage: -80 °C

Storage Comment: Store in working aliquots at -70 °C.

Target Details

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Alternative Name: ATG7 (ATG7 Products)

Background: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2). This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1 (3). Another member of the autophagy family of proteins is APG7 which was identified in yeast as a ubiquitin-E1-like enzyme , this function is conserved in the mammalian homolog (4). In mammalian cells, APG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles (5). It has been suggested that caspase-8 may alter APG7 levels and thus the APG7 program of autophagic cell death (6).

Molecular Weight: 30 kDa (Calculated)

Gene ID: 74244

OMIM: 37589293

UniProt: Q9D906

Pathways: Response to Water Deprivation, Autophagy