ATG7 Protein (AA 1-703) (GST tag)

Catalog No. ABIN1345982

Product Details

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Protein Type: Recombinant

Protein Characteristics: AA 1-703

Origin: Human

Source: Wheat germ

Purification tag / Conjugate: This ATG7 protein is labelled with GST tag.

Application: Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)

Purpose: ATG7 (Human) Recombinant Protein (P01)

Sequence: MAAATGDPGL SKLQFAPFSS ALDVGFWHEL TQKKLNEYRL DEAPKDIKGY YYNGDSAGLP ARLTLEFSAF DMSAPTPARC CPAIGTLYNT NTLESFKTAD KKLLLEQAAN EIWESIKSGT ALENPVLLNK FLLLTFADLK KYHFYYWFCY PALCLPESLP LIQGPVGLDQ RFSLKQIEAL ECAYDNLCQT EGVTALPYFL IKYDENMVLV SLLKHYSDFF QGQRTKITIG VYDPCNLAQY PGWPLRNFLV LAAHRWSSSF QSVEVVCFRD RTMQGARDVA HSIIFEVKLP EMAFSPDCPK AVGWEKNQKG GMGPRMVNLS ECMDPKRLAE SSVDLNLKLM CWRLVPTLDL DKVVSVKCLL LGAGTLGCNV ARTLMGWGVR HITFVDNAKI SYSNPVRQPL YEFEDCLGGG KPKALAAADR LQKIFPGVNA RGFNMSIPMP GHPVNFSSVT LEQARRDVEQ LEQLIESHDV VFLLMDTRES RWLPAVIAAS KRKLVINAAL GFDTFVVMRH GLKKPKQQGA GDLCPNHPVA SADLLGSSLF ANIPGYKLGC YFCNDVVAPG DSTRDRTLDQ QCTVSRPGLA VIAGALAVEL MVSVLQHPEG GYAIASSSDD RMNEPPTSLG LVPHQIRGFL SRFDNVLPVS LAFDKCTACS SKVLDQYERE GFNFLAKVFN SSHSFLEDLT GLTLLHQETQ AAEIWDMSDD ETI

Characteristics: Human ATG7 full-length ORF ( NP_006386.1, 1 a.a. - 703 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Han, Hou, Goldstein, Stolz, Watkins, Rabinowich: "A Complex between Atg7 and Caspase-9: A NOVEL MECHANISM OF CROSS-REGULATION BETWEEN AUTOPHAGY AND APOPTOSIS." in: The Journal of biological chemistry, Vol. 289, Issue 10, pp. 6485-97, (2014) (PubMed).

Target Details

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Alternative Name: ATG7 (ATG7 Products)

Synonyms: APG7-LIKE Protein, APG7L Protein, GSA7 Protein, D8 Protein, UBA1B Protein, UBE1L Protein, UBE2 Protein, Ube1l Protein, 1300004C08Rik Protein, AO090001000744 Protein, atg7 Protein, 1810013K23Rik Protein, Agp7 Protein, Apg7l Protein, Atg7l Protein, ATAPG7 Protein, ATATG7 Protein, ATG7 Protein, AUTOPHAGY 7 Protein, AUTOPHAGY-RELATED 7 Protein, K15I22.10 Protein, K15I22_10 Protein, autophagy related 7 Protein, ubiquitin like modifier activating enzyme 7 Protein, ubiquitin-like modifier activating enzyme 7 Protein, autophagy-related protein 7 Protein, Autophagy-related protein 7 Protein, Apg7p Protein, ThiF family protein Protein, ubiquitin-like conjugating enzyme Atg7 Protein, ATG7 Protein, UBA7 Protein, Uba7 Protein, ANI_1_3368024 Protein, AOR_1_1338164 Protein, PTRG_02236 Protein, PAAG_00287 Protein, PITG_12537 Protein, atg7 Protein, Atg7 Protein, APG7 Protein

Background: Full Gene Name: ATG7 autophagy related 7 homolog (S. cerevisiae)
Synonyms: APG7-LIKE,APG7L,DKFZp434N0735,GSA7

Gene ID: 10533

NCBI Accession: NM_006395

Pathways: Response to Water Deprivation, Autophagy