Acyl-CoA Synthetase Long-Chain Family Member 3 (Acsl3) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)

Details for Product No. ABIN2714345
Protein Name
Protein Characteristics
Transcript Variant 1
3
2
1
1
Origin
Human
5
2
Source
HEK-293 Cells
4
2
1
Protein Type
Recombinant
Purification tag / Conjugate
This Acsl3 protein is labelled with Myc-DYKDDDDK Tag.
Application
Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
Options
Specificity Optimal preservation of protein structure, post-translational modifications and functions.
Characteristics
  • Recombinant human ACSL3 (transcript variant 1) protein expressed in HEK293 cells.
  • Produced with end-sequenced ORF clone
Purity > 80 % as determined by SDS-PAGE and Coomassie blue staining
Protein Name
Background The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in brain, and preferentially utilizes myristate, arachidonate, and eicosapentaenoate as substrates. The amino acid sequence of this isozyme is 92 % identical to that of rat homolog. Two transcript variants encoding the same protein have been found for this gene.
Molecular Weight 80.2 kDa
NCBI Accession NP_004448
Pathways SARS-CoV-2 Protein Interactome
Application Notes Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays
Comment

The tag is located at the C-terminal.

Restrictions For Research Use only
Concentration > 50 μg/mL
Buffer 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
Storage -80 °C
Storage Comment Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
Supplier Images
Image no. 1 for Acyl-CoA Synthetase Long-Chain Family Member 3 (Acsl3) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag) (ABIN2714345) Validation with Western Blot