Neurturin (NRTN) (Active) Protein

Details for Product No. ABIN2727208
Protein Name
Origin
Human
9
1
Source
Escherichia coli (E. coli)
8
2
Protein Type
Recombinant
Biological Activity
Active
Application
Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
Options
Specificity Optimal preservation of protein structure, post-translational modifications and functions.
Characteristics
  • Recombinant human Neurturin protein expressed in E. coli.
  • Produced with end-sequenced ORF clone
  • Tested for bioactivity.
Purity > 95 % as determined by SDS-PAGE and Coomassie blue staining
Endotoxin Level Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
Biological Activity Comment Human Neurturin at a concentration of 100 ng/ml will support the survival of 65% of newborn rat sympathetic neurons.
Protein Name
Background This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. The encoded preproprotein is proteolytically processed to generate the mature protein. This protein signals through the RET receptor tyrosine kinase and a GPI-linked coreceptor, and promotes survival of neuronal populations. A neurturin mutation has been described in a family with Hirschsprung Disease.
Molecular Weight 11.8 kDa
NCBI Accession NP_004549
Application Notes Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays
Restrictions For Research Use only
Buffer Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
Handling Advice Resuspend the protein in the desired concentration in proper buffer
Storage -80 °C
Storage Comment Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.