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N-terminal Asparagine Amidase (NTAN1) protein (Myc-DYKDDDDK Tag) Protein

NTAN1 Origin: Human Source: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, Func, PI, STD
Catalog No. ABIN2727674
$888.80
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20 μg
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  • Protein Name
    Protein Type
    Recombinant
    Origin
    Human
    Source
    HEK-293 Cells
    Purification tag / Conjugate
    Myc-DYKDDDDK Tag
    Application
    Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
    Specificity
    Optimal preservation of protein structure, post-translational modifications and functions.
    Characteristics
    • Recombinant human NTAN1 protein expressed in HEK293 cells.
    • Produced with end-sequenced ORF clone
    Purity
    > 80 % as determined by SDS-PAGE and Coomassie blue staining
  • Protein Name
    Background
    The protein encoded by this gene functions in a step-wise process of protein degradation through the N-end rule pathway. This protein acts as a tertiary destabilizing enzyme that deamidates N-terminal L-Asn residues on proteins to produce N-terminal L-Asp. L-Asp substrates are subsequently conjugated to L-Arg, which is recognized by specific E3 ubiquitin ligases and targeted to the proteasome. Pseudogenes of this gene are located on the long arms of chromosomes 8, 10 and 12. Alternative splicing results in multiple transcript variants that encode different protein isoforms.
    Molecular Weight
    34.5 kDa
    NCBI Accession
    NP_775745
  • Application Notes
    Recombinant human proteins can be used for:
    Native antigens for optimized antibody production
    Positive controls in ELISA and other antibody assays
    Protein-protein interaction
    In vitro biochemical assays and cell-based functional assays
    Comment

    The tag is located at the C-terminal.

    Restrictions
    For Research Use only
  • Concentration
    > 50 μg/mL
    Buffer
    25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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