N-terminal Asparagine Amidase (NTAN1) protein (Myc-DYKDDDDK Tag) Protein
- Protein Name
- Protein Type
- HEK-293 Cells
- Purification tag / Conjugate
- Myc-DYKDDDDK Tag
- Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
- Optimal preservation of protein structure, post-translational modifications and functions.
- Recombinant human NTAN1 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Protein Name
- The protein encoded by this gene functions in a step-wise process of protein degradation through the N-end rule pathway. This protein acts as a tertiary destabilizing enzyme that deamidates N-terminal L-Asn residues on proteins to produce N-terminal L-Asp. L-Asp substrates are subsequently conjugated to L-Arg, which is recognized by specific E3 ubiquitin ligases and targeted to the proteasome. Pseudogenes of this gene are located on the long arms of chromosomes 8, 10 and 12. Alternative splicing results in multiple transcript variants that encode different protein isoforms.
- Molecular Weight
- 34.5 kDa
- NCBI Accession
- Application Notes
- Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
In vitro biochemical assays and cell-based functional assays
The tag is located at the C-terminal.
- For Research Use only
- > 50 μg/mL
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.