RAD51 Homolog C (S. Cerevisiae) (RAD51C) protein (His tag)

Details for Product No. ABIN2730411
Protein Name
Origin
Human
2
1
1
1
Source
Escherichia coli (E. coli)
2
2
1
Protein Type
Recombinant
Purification tag / Conjugate
This RAD51C protein is labelled with His tag.
Application
Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
Options
Specificity Optimal preservation of protein structure, post-translational modifications and functions.
Characteristics
  • Recombinant human RAD51C (full length, N-term HIS tag, transcript variant 2) protein expressed in E. coli.
  • Produced with end-sequenced ORF clone
Purity > 80 % as determined by SDS-PAGE and Coomassie blue staining
Protein Name
Background This gene is a member of the RAD51 family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51 and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with other RAD51 paralogs and is reported to be important for Holliday junction resolution. Mutations in this gene are associated with Fanconi anemia-like syndrome. This gene is one of four localized to a region of chromosome 17q23 where amplification occurs frequently in breast tumors. Overexpression of the four genes during amplification has been observed and suggests a possible role in tumor progression. Alternative splicing results in multiple transcript variants.
Molecular Weight 14.7 kDa
NCBI Accession NP_002867
Pathways DNA Damage Repair
Application Notes Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays
Comment

The tag is located at the N-terminal.
Restrictions For Research Use only
Concentration > 50 μg/mL
Buffer 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.
Storage -80 °C
Storage Comment Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
Supplier Images
Image no. 1 for RAD51 Homolog C (S. Cerevisiae) (RAD51C) protein (His tag) (ABIN2730411) Validation with Western Blot