FGF1 Protein (AA 16-155)

Catalog No. ABIN2745668

Product Details

Target: FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))

Protein Type: Recombinant

Protein Characteristics: AA 16-155

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purpose: FGF-1 (mouse) (rec.) (untagged)

Cross-Reactivity: Mouse

Characteristics: Mouse FGF-1 (aa 16-155) is untagged.

Purity: >95 % (SDS-PAGE)

Endotoxin Level: <0.01EU/μg purified protein (LAL test).

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 100 μL sterile water.

Concentration: 0.1 mg/mL

Buffer: In PBS containing 0.2 mM DTT.

Handling Advice: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 6 months

Target Details

Target: FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))

Alternative Name: FGF-1 (FGF1 Products)

Background:

Fibroblast Growth Factor 1, Acidic Fibroblast Growth Factor, Endothelial Cell Growth Factor, ECGF, Heparin-binding Growth Factor 1, HBGF-1

Fibroblast growth factors (FGFs) constitute a family of heparin-binding polypeptides involved in the regulation of biological responses such as growth, differentiation and angiogenesis. The biological effects of FGFs are mediated by four structurally related receptor tyrosine kinases denoted FGFR1, FGFR2, FGFR3 and FGFR4. FGF-1 [FGF-acidic, ECGF, HBGF-1] is a powerful mitogen of cells of mesodermal, ectodermal and endodermal origin. FGF-1 association with heparan sulfate is a prerequisite for activation of FGF receptors. FGF-1 plays a role in various stages of development and morphogenesis as well as in angiogenesis and wound healing processes. Recent data indicate a role of FGF-1 in inflammation and obesity. FGF-1 is selectively induced in fat cells by high-fat diet feeding and established the PPARgamma-FGF-1 axis as a critical pathway that regulates adipose tissue remodeling.

Molecular Weight: ~16kDa

UniProt: P61148

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway