FGF1 Protein (AA 16-155, Isoform 1)

Catalog No. ABIN2017849

Product Details

Target: FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: Isoform 1, AA 16-155

Origin: Human

Source: Escherichia coli (E. coli)

Characteristics: ED50<0.3 ng/mL, measured by a cell proliferation assay of 3T3 Cells, corresponding to a specific activity of >3.3 × 10^6 IU/mg in the presence of 10 μg/mL of heparin.

Purity: > 95 % by SDS-PAGE and HPLC analyses.

Endotoxin Level: < 0.2 EU/μg, determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O or PBS at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Storage: -80 °C

Storage Comment: Lyophilized recombinant human Fibroblast Growth Factor- acidic (rhFGF-acidic) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhFGF-acidic should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.

Expiry Date: 6 months

Target Details

Target: FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))

Alternative Name: Fibroblast Growth Factor-Acidic(FGF-Acidic) (FGF1 Products)

Synonyms: AFGF Protein, ECGF Protein, ECGF-beta Protein, ECGFA Protein, ECGFB Protein, FGF-1 Protein, FGF-alpha Protein, FGFA Protein, GLIO703 Protein, HBGF-1 Protein, HBGF1 Protein, Fgf1 Protein, FGF1 Protein, aFGF Protein, ecgf Protein, fgfa Protein, ecgfa Protein, ecgfb Protein, fgf-1 Protein, hbgf1 Protein, glio703 Protein, ecgf-beta Protein, fgf-alpha Protein, Dffrx Protein, Fam Protein, Fgf-1 Protein, Fgfa Protein, zgc:136885 Protein, LOC100221393 Protein, fgf1 Protein, zgc:73249 Protein, fibroblast growth factor 1 Protein, fibroblast growth factor 1 L homeolog Protein, fibroblast growth factor 1b Protein, fibroblast growth factor 1a Protein, FGF1 Protein, fgf1.L Protein, fgf1 Protein, Fgf1 Protein, fgf1b Protein, LOC100221393 Protein, fgf1a Protein

Background: Fibroblast Growth Factor- acidic (FGF-acidic), also known as FGF-1 and endothelial cell growth factor, is a member of the FGF family which currently contain 23 members. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway. FGF acidic has been detected in large amounts in the brain. Other cells known to express FGF acidic include hepatocytes, vascular smooth muscle cells, CNS neurons, skeletal muscle cells, fibroblasts, keratinocytes, endothelial cells, intestinal columnar epithelium cells and pituitary basophils and acidophils. As with other FGF's, FGF acidic exhibits considerable species cross reactivity. FGF acidic and FGF basic stimulate the proliferation of all cells of mesodermal origin, and many cells of neuroectodermal, ectodermal and endodermal origin. Recombinant human Fibroblast Growth Factor- acidic (rhFGF-acidic)produced in E. coli is a single non-glycosylated polypeptide chain containing 140 amino acids. A fully biologically active molecule, rhFGF-acidic has a molecular mass of 15.8 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques.
Synonyms: HBGF-1, ECGF-beta, FIBP, FGFIBP, FIBP-1, ECGF, ECGFA, GLIO703, FGF1, FGF-a

Molecular Weight: 15.8 kDa, observed by reducing SDS-PAGE.

UniProt: P05230

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway