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MYT1 Protein (AA 1-1121) (Strep Tag)

MYT1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC). SDS, WB, ELISA
Catalog No. ABIN3094003
  • Target See all MYT1 Proteins
    MYT1 (Myelin Transcription Factor 1 (MYT1))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-1121
    Origin
    • 3
    • 2
    Human
    Source
    • 3
    • 1
    • 1
    Tobacco (Nicotiana tabacum)
    Purification tag / Conjugate
    This MYT1 protein is labelled with Strep Tag.
    Application
    SDS-PAGE (SDS), Western Blotting (WB), ELISA
    Sequence
    MSLENEDKRA RTRSKALRGP PETTAADLSC PTPGCTGSGH VRGKYSRHRS LQSCPLAKKR KLEGAEAEHL VSKRKSHPLK LALDEGYGVD SDGSEDTEVK DASVSDESEG TLEGAEAETS GQDEIHRPET AEGRSPVKSH FGSNPIGSAT ASSKGSYSSY QGIIATSLLN LGQIAEETLV EEDLGQAAKP GPGIVHLLQE AAEGAASEEG EKGLFIQPED AEEVVEVTTE RSQDLCPQSL EDAASEESSK QKGILSHEEE DEEEEEEEEE EEEDEEEEEE EEEEEEEEEE EEEEEEEEEE EEEEEEAAPD VIFQEDTSHT SAQKAPELRG PESPSPKPEY SVIVEVRSDD DKDEDTHSRK STVTDESEMQ DMMTRGNLGL LEQAIALKAE QVRTVCEPGC PPAEQSQLGL GEPGKAAKPL DTVRKSYYSK DPSRAEKREI KCPTPGCDGT GHVTGLYPHH RSLSGCPHKD RIPPEILAMH ENVLKCPTPG CTGQGHVNSN RNTHRSLSGC PIAAAEKLAK SHEKQQPQTG DPSKSSSNSD RILRPMCFVK QLEVPPYGSY RPNVAPATPR ANLAKELEKF SKVTFDYASF DAQVFGKRML APKIQTSETS PKAFQCFDYS QDAEAAHMAA TAILNLSTRC WEMPENLSTK PQDLPSKSVD IEVDENGTLD LSMHKHRKRE NAFPSSSSCS SSPGVKSPDA SQRHSSTSAP SSSMTSPQSS QASRQDEWDR PLDYTKPSRL REEEPEESEP AAHSFASSEA DDQEVSEENF EERKYPGEVT LTNFKLKFLS KDIKKELLTC PTPGCDGSGH ITGNYASHRS LSGCPLADKS LRNLMAAHSA DLKCPTPGCD GSGHITGNYA SHRSLSGCPR AKKSGVKVAP TKDDKEDPEL MKCPVPGCVG LGHISGKYAS HRSASGCPLA ARRQKEGSLN GSSFSWKSLK NEGPTCPTPG CDGSGHANGS FLTHRSLSGC PRATFAGKKG KLSGDEVLSP KFKTSDVLEN DEEIKQLNQE IRDLNESNSE MEAAMVQLQS QISSMEKNLK NIEEENKLIE EQNEALFLEL SGLSQALIQS LANIRLPHME PICEQNFDAY VSTLTDMYSN QDPENKDLLE SIKQAVRGIQ V
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    Characteristics
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified in one-step affinity chromatography
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.


    Expression System:
    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured against its specific reference buffer.
    • We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

    Purification
    One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
    Purity
    > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
    Top Product
    Discover our top product MYT1 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    The buffer composition is at the discretion of the manufacturer.
    Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    12 months
  • Target
    MYT1 (Myelin Transcription Factor 1 (MYT1))
    Alternative Name
    MYT1 (MYT1 Products)
    Background
    Myelin transcription factor 1 (MyT1) (Myelin transcription factor I) (MyTI) (PLPB1) (Proteolipid protein-binding protein),FUNCTION: Binds to the promoter region of genes encoding proteolipid proteins of the central nervous system. May play a role in the development of neurons and oligodendroglia in the CNS. May regulate a critical transition point in oligodendrocyte lineage development by modulating oligodendrocyte progenitor proliferation relative to terminal differentiation and up-regulation of myelin gene transcription. {ECO:0000269|PubMed:14962745}.
    Molecular Weight
    122.3 kDa
    UniProt
    Q01538
    Pathways
    Cell Division Cycle
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