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Synaptotagmin VI (SYT6) (AA 1-510) protein (rho-1D4 tag) Protein

SYT6 Origin: Human Source: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Catalog No. ABIN3115444
$5,122.60
Plus shipping costs $45.00 and $22.00 dry ice
0.25 mg ABIN3115444
0.25 mg ABIN3115444
local_shipping Shipping to: United States
Delivery in 50 to 55 Business Days
  • Protein Name
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-510
    Origin
    Human
    Source
    Insect Cells
    Purification tag / Conjugate
    rho-1D4 tag
    Application
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequence
    MSGVWGAGGP RCQEALAVLA SLCRARPPPL GLDVETCRSF ELQPPERSPS AAGAGTSVSL LAVVVIVCGV ALVAVFLFLF WKLCWMPWRN KEASSPSSAN PPLEALQSPS FRGNMADKLK DPSTLGFLEA AVKISHTSPD IPAEVQMSVK EHIMRHTRLQ RQTTEPASST RHTSFKRHLP RQMHVSSVDY GNELPPAAEQ PTSIGRIKPE LYKQKSVDGE DAKSEATKSC GKINFSLRYD YETETLIVRI LKAFDLPAKD FCGSSDPYVK IYLLPDRKCK LQTRVHRKTL NPTFDENFHF PVPYEELADR KLHLSVFDFD RFSRHDMIGE VILDNLFEAS DLSRETSIWK DIQYATSESV DLGEIMFSLC YLPTAGRLTL TVIKCRNLKA MDITGYSDPY VKVSLLCDGR RLKKKKTTIK KNTLNPVYNE AIIFDIPPEN MDQVSLLISV MDYDRVGHNE IIGVCRVGIT AEGLGRDHWN EMLAYPRKPI AHWHSLVEVK KSFKEGNPRL
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human SYT6 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
    1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
    2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
    3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin-free.
    Grade
    Crystallography grade
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Protein Name
    Background
    May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. May mediate Ca(2+)-regulation of exocytosis in acrosomal reaction in sperm (By similarity). {ECO:0000250|UniProtKB:Q9R0N8}.
    Molecular Weight
    58.5 kDa Including tag.
    UniProt
    Q5T7P8
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