FOXN1 Protein (AA 1-648) (Strep Tag)

Catalog No. ABIN3135432

Product Details

Target: FOXN1 (Forkhead Box N1 (FOXN1))

Protein Type: Recombinant

Protein Characteristics: AA 1-648

Origin: Mouse

Source: Cell-free protein synthesis (CFPS)

Purification tag / Conjugate: This FOXN1 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Brand: AliCE®

Sequence: MVSLLPPQSD VTLPGSTRLE GEPQGDLMQA PGLPDSPAPQ NKHANFSCSS FVPDGPPERT PSLPPHSPSI ASPDPEQIQG HCTAGPGPGS FRLSPSEKYP GFGFEEGPAG SPGRFLKGNH MPFHPYKRHF HEDIFSEAQT AMALDGHSFK TQGALEAFEE IPVDMGDAEA FLPSFPAEAW CNKLPYPSQE HNQILQGSEV KVKPQALDSG PGMYCYQPPL QHMYCSSQPA FHQYSPGGGS YPVPYLGSPH YPYQRIAPQA NAEGHQPLFP KPIYSYSILI FMALKNSKTG SLPVSEIYNF MTEHFPYFKT APDGWKNSVR HNLSLNKCFE KVENKSGSSS RKGCLWALNP SKIDKMQEEL QKWKRKDPIA VRKSMAKPEE LDSLIGDKRE KLGSPLLGCP PPGLAGPGPI RPMAPSAGLS QPLHPMHPAP GPMPGKNPLQ DLLGGHAPSC YGQTYPHLSP SLAPSGHQQP LFPQPDGHLE LQAQPGTPQD SPLPAHTPPS HGAKLMAEPS SARTMHDTLL PDGDLGTDLD AINPSLTDFD FQGNLWEQLK DDSLALDPLV LVTSSPTSSS MLPPPPAAHC FPPGPCLAET GNEAGELAPP GSGGSGALGD MHLSTLYSAF VELESTPSSA AAGPAVYLSP GSKPLALA
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).

Purity: > 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: FOXN1 (Forkhead Box N1 (FOXN1))

Alternative Name: Foxn1 (FOXN1 Products)

Background: Forkhead box protein N1 (Hepatocyte nuclear factor 3 forkhead homolog 11) (HFH-11) (HNF-3/forkhead homolog 11) (Winged-helix transcription factor nude),FUNCTION: Transcriptional regulator which regulates the development, differentiation, and function of thymic epithelial cells (TECs) both in the prenatal and postnatal thymus. Acts as a master regulator of the TECs lineage development and is required from the onset of differentiation in progenitor TECs in the developing fetus to the final differentiation steps through which TECs mature to acquire their full functionality. Regulates, either directly or indirectly the expression of a variety of genes that mediate diverse aspects of thymus development and function, including MHC Class II, DLL4, CCL25, CTSL, CD40 and PAX1. Regulates the differentiation of the immature TECs into functional cortical TECs (cTECs) and medullary TECs (mTECs) (PubMed:22072979). Essential for maintenance of mTECs population in the postnatal thymus (PubMed:19955175). Involved in the morphogenesis and maintenance of the three-dimensional thymic microstructure which is necessary for a fully functional thymus (PubMed:21109991). Plays an important role in the maintenance of hematopoiesis and particularly T lineage progenitors within the bone marrow niche with age (PubMed:24184560). Essential for the vascularization of the thymus anlage (PubMed:19853842). Promotes the terminal differentiation of epithelial cells in the epidermis and hair follicles, partly by negatively regulating the activity of protein kinase C (PubMed:17459087). {ECO:0000269|PubMed:17459087, ECO:0000269|PubMed:19853842, ECO:0000269|PubMed:19955175, ECO:0000269|PubMed:21109991, ECO:0000269|PubMed:22072979, ECO:0000269|PubMed:24184560}.

Molecular Weight: 69.2 kDa

UniProt: Q61575