Angiopoietin 1 Protein (ANGPT1) (AA 284-498) (DYKDDDDK Tag)

Catalog No. ABIN5564294

Product Details

Target: Angiopoietin 1 (ANGPT1)

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 284-498

Origin: Human

Source: CHO Cells

Purification tag / Conjugate: This Angiopoietin 1 protein is labelled with DYKDDDDK Tag.

Application: SDS-PAGE (SDS)

Purpose: COMP (rat):Angiopoietin-1 (human) (rec.)

Specificity: Binds to human angiopoietin-1 receptor (Tie-2).

Cross-Reactivity: Human

Characteristics: The fibrinogen-like domain (aa 284-498) of human angiopoietin-1 is fused at the N-terminus to the coiled-coil domain of rat COMP (cartilage oligomeric matrix protein) and to a FLAG®-tag.

Purity: >95 % (SDS-PAGE)

Endotoxin Level: <0.05EU/μg purified protein (LAL test).

Biological Activity Comment: More potent than native angiopoietin-1. The protein forms pentameric structures.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Contains PBS + 0.02 % CHAPS.

Handling Advice: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 6 months

Target Details

Target: Angiopoietin 1 (ANGPT1)

Alternative Name: Angiopoietin-1 (ANGPT1 Products)

Background:

COMP-Ang1, COMP:Ang-1, COMP Angiopoietin 1, COMP ANGPT1

Soluble, stable and more potent angiopoietin-1 (Ang-1) variant.

Molecular Weight: 35kDa (SDS-PAGE)

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process