COL3A1 Protein

Catalog No. ABIN5624594

Supplier Product No.: 009-001-105

Product Details

Target: COL3A1 (Collagen, Type III, alpha 1 (COL3A1))

Protein Type: Native

Origin: Human

Source: Human

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC)

Supplier Product No.: 009-001-105

Supplier: Rockland

Purpose: Human Collagen Type III

Characteristics: Type III collagen, collagen 3, human collagen, Alpha 1 type III collagen, Alpha1 (III) collagen, COL3A1_HUMAN, Collagen Type III Alpha 1 Chain, Ehlers-Danlos Syndrome Type IV Autosomal Dominant, Fetal Collagen, Human placenta Collagen Type III Protein, Human Type III Collagen Protein, scleroprotein collagen

Purification: Human Collagen Type III has been prepared from human placenta and is chromatographically and immunologically pure. This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins.  This product reacts with Anti-Collagen Type III.  Reaction with Rockland's Anti-Collagen I, II, IV, V or VI is negligible.

Sterility: Sterile filtered

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:100-1:500

Application Note: Human Collagen Type III purified protein standard has been tested in SDS-Page and western blot and is used as a control or standard in indirect trapping ELISA for quantitation of antigen using a standard curve, for immunoprecipitation, and for other immunological assays as optimized by the user. Specific conditions should be optimized by user. Collagen Type III is recognized by type specific anti-collagen antibodies that recognize a native three-dimensional structure.

Western Blot Dilution: 1:1000

Immunoprecipitation Dilution: 1:100-1:500

ELISA Dilution: 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.5 M Acetic Acid

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 6 months

Target Details

Target: COL3A1 (Collagen, Type III, alpha 1 (COL3A1))

Alternative Name: COL3A1 (COL3A1 Products)

Background: Background: In muscle tissue, collagen serves as a major component of the endomysium. Collagen constitutes one to two percent of muscle tissue, and accounts for 6 % of the weight of strong, tendinous muscles. A collagen may be defined as a protein containing sizable domain(s) of triple-helical conformation. Collagen type III, a fibrous scleroprotein found in bone, cartilage, tendon, bone marrow stroma and other connective tissue, yields gelatin on boiling. Collagen Type III scleroprotein is a simple protein found in horny and cartilaginous tissues and in the lens of the eye.

Gene ID: 1281

NCBI Accession: NP_000081

UniProt: P02461

Pathways: Autophagy, Growth Factor Binding