IL16 Protein (AA 1203-1332) (His tag)
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- Target See all IL16 Proteins
- IL16 (Interleukin 16 (IL16))
- Biological Activity
- Active
- Protein Characteristics
- AA 1203-1332
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This IL16 protein is labelled with His tag.
- Application
- Cell Culture (CC), Activity Assay (AcA)
- Characteristics
- Tag location: N-terminal His Tag
- Purity
- > 97 %
- Biological Activity Comment
- Pro-IL16 (Interleukin16) is a 631 amino acid precursor molecule, which is then cleaved into different isoforms. Researches have shown that the recombinant human IL16, containing C-terminal 130 amino acids, has same bioactivity as the natural secreted human IL16. Besides, IL16 has been considered to stimulate the proliferation of Jurkat cells at low dose (10-9 M). Thus, a proliferation assay of recombinant human IL16 was conducted using Jurkat cells. Briefly, Jurkat cells were seeded into triplicate wells of 96-well plates at a density of 10, 000 cells/well in RPMI-1640 with the addition of various concentrations of IL16. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Cell proliferation of Jurkat cells after incubation with IL16 for 72h observed by inverted microscope was shown in Figure 1. The CCK-8 data was shown in Figure 2. It was obvious that IL16 significantly promoted cell proliferation of Jurkat cells.
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Interleukin 16 (IL16) (Active) protein
IL16 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant SDS, Func Active
Interleukin 16 (IL16) (AA 1203-1332) (Active) protein (His tag)IL16 Origin: Human Host: Escherichia coli (E. coli) > 97 % CC, AcA Active
Interleukin 16 (IL16) (AA 1212-1332) protein (His-IF2DI Tag)IL16 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Interleukin 16 (IL16) (AA 1-454) protein (GST tag)IL16 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Interleukin 16 (IL16) (AA 1212-1332) protein (His tag)Crystallography grade IL16 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Interleukin 16 (IL16) (AA 1205-1322) protein (His tag)Crystallography grade IL16 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Interleukin 16 (IL16) (AA 1-1332) protein (Strep Tag)Crystallography grade IL16 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Interleukin 16 (IL16) (AA 1-1322) protein (Strep Tag)Crystallography grade IL16 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
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- Application Notes
- Isoelectric Point: 6
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- Preservative
- Dithiothreitol (DTT), Other preservative, ProClin
- Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- Target
- IL16 (Interleukin 16 (IL16))
- Abstract
- IL16 Products
- Synonyms
- IL16 Protein, LCF Protein, NIL16 Protein, PRIL16 Protein, prIL-16 Protein, mKIAA4048 Protein, IL-16 Protein, interleukin 16 Protein, IL16 Protein, Il16 Protein
- Background
- Alternative Names: LCF, PrIL-16, Lymphocyte Chemoattractant Factor, Pro-Interleukin 16
- Molecular Weight
- 17kDa
- UniProt
- Q14005
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