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Interleukin 16 (IL16) (AA 1203-1332) (Active) protein (His tag) Protein

IL16 Origin: Human Host: Escherichia coli (E. coli) > 97 % AcA, CC Active
Catalog No. ABIN5665840
$5,400.00
Plus shipping costs $45.00
1 mg ABIN5665840
1 mg ABIN5665840
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Delivery in 12 to 15 Business Days
  • Target
    IL16
    Biological Activity
    Active
    Protein Characteristics
    AA 1203-1332
    Origin
    Human
    Source
    Escherichia coli (E. coli)
    • 61
    • 2
    • 1
    • 1
    Purification tag / Conjugate
    This IL16 protein is labelled with His tag.
    Application
    Activity Assay (AcA), Cell Culture (CC)
    Characteristics
    Tag location: N-terminal His Tag
    Purity
    > 97 %
    Biological Activity Comment
    Pro-IL16 (Interleukin16) is a 631 amino acid precursor molecule, which is then cleaved into different isoforms. Researches have shown that the recombinant human IL16, containing C-terminal 130 amino acids, has same bioactivity as the natural secreted human IL16. Besides, IL16 has been considered to stimulate the proliferation of Jurkat cells at low dose (10-9 M). Thus, a proliferation assay of recombinant human IL16 was conducted using Jurkat cells. Briefly, Jurkat cells were seeded into triplicate wells of 96-well plates at a density of 10, 000 cells/well in RPMI-1640 with the addition of various concentrations of IL16. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Cell proliferation of Jurkat cells after incubation with IL16 for 72h observed by inverted microscope was shown in Figure 1. The CCK-8 data was shown in Figure 2. It was obvious that IL16 significantly promoted cell proliferation of Jurkat cells.
  • Application Notes
    Isoelectric Point: 6
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
    Preservative
    Dithiothreitol (DTT), Other preservative, ProClin
    Precaution of Use
    This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
  • Target
    IL16
    Synonyms
    IL16, LCF, NIL16, PRIL16, prIL-16, mKIAA4048, IL-16, interleukin 16, IL16, Il16
    Background
    Alternative Names: LCF, PrIL-16, Lymphocyte Chemoattractant Factor, Pro-Interleukin 16
    Molecular Weight
    17kDa
    UniProt
    Q14005
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