LTA Protein (AA 35-205) (His tag)
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- Target See all LTA Proteins
- LTA (Lymphotoxin-alpha (LTA))
- Biological Activity
- Active
- Protein Characteristics
- AA 35-205
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This LTA protein is labelled with His tag.
- Application
- Activity Assay (AcA), Cell Culture (CC)
- Characteristics
- Tag location: N-terminal His Tag
- Purity
- > 97 %
- Biological Activity Comment
- Mechanism: TNF-β, a member of the tumor necrosis factor family, is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells. The biological effects of TNF-β are very similar to TNF-α, due to the similarity of molecular structure and the receptors. As reported, TNF-α could inhibit the proliferation and induce apoptosis of A549 cells, and the concentration of IL-1β in cell supernatant will increase after stimulation. Therefore, A549 cells were incubated in DMEM with TNF-β (10ng/mL) for 8h, 24h, 48h, 72h, then cells were observed by inverted microscope and IL-1β was detected in the cell supernatant by ELISA . Results 1: Cell apoptosis was observed after incubation with TNF-β (10ng/mL) for 72h.
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Lymphotoxin-alpha (LTA) (Active) protein
LTA Origin: Chemical Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
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Lymphotoxin-alpha (LTA) (AA 34-202) protein (His tag)LTA Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Lymphotoxin-alpha (LTA) (AA 35-205) protein (His tag)LTA Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Lymphotoxin-alpha (LTA) (AA 1-205) protein (GST tag)LTA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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- Application Notes
- Isoelectric Point: 9.3
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- Preservative
- Dithiothreitol (DTT), Other preservative, ProClin
- Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- Target
- LTA (Lymphotoxin-alpha (LTA))
- Alternative Name
- Tumor Necrosis Factor Beta (TNFb) (LTA Products)
- Target Type
- Chemical
- Background
- Alternative Names: LT, LTA, TNF-B, TNFSF1, Tumor Necrosis Factor Ligand Superfamily Member 1, Lymphotoxin Alpha
- Molecular Weight
- 23kDa
- UniProt
- P01374
- Pathways
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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