MMP2 Protein (AA 110-660) (His tag)
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- Target See all MMP2 Proteins
- MMP2 (Matrix Metalloproteinase 2 (MMP2))
- Biological Activity
- Active
- Protein Characteristics
- AA 110-660
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This MMP2 protein is labelled with His tag.
- Application
- Activity Assay (AcA), Cell Culture (CC)
- Characteristics
- Tag location: N-terminal His Tag
- Purity
- > 97 %
- Biological Activity Comment
- Mechanism: MMP2 is a zinc-dependent enzymes capable of cleaving components of the extracellular matrix, which belongs to the matrix metalloproteinase (MMP) family. It is a gelatinase A, 72kDa type IV collagenase which can hydrolyze gelatin under certain conditions. Gelatin zymography is mainly used for the detection of the gelatinases, MMP-2 and MMP-9 and It is extremely sensitive because levels of 10pg of MMP-2 can already be detected. Briefly, various concentrations of MMP2 (500ng, 125ng, 63ng, 32ng, 16ng) were denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphate-polyacrylamide gel (SDS-PAGE; 10% gels) containing gelatin (1mg/mL) with nonreducing conditions. After renaturation, incubation and CCB-stained, active MMP2 would hydrolyze gelatin nearby, which was indicated by the white binds on the gel. In this experiment we use heat-denatured MMP2 protein as negative control, and blood sample as positive control. Result 1: Gelatin hydrolysis by recombinant human MMP2 was shown in figure 1. MMP2 MMP2 MMP2 MMP2 MMP2 negative positive 500ng 125ng 63ng 32ng 16ng control control 70kd→ Figure 1. Hydrolysis of gelatin by MMP2.
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Matrix Metalloproteinase 2 (MMP2) (Active) protein
MMP2 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Matrix Metalloproteinase 2 (MMP2) (AA 34-660) protein (His tag)MMP2 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Matrix Metalloproteinase 2 (MMP2) (AA 424-660) protein (His-IF2DI Tag)MMP2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Matrix Metalloproteinase 2 (MMP2) (AA 1-660) protein (GST tag)MMP2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Matrix Metalloproteinase 2 (MMP2) (AA 109-660) protein (His tag)MMP2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Matrix Metalloproteinase 2 (MMP2) (AA 30-660) protein (His tag)MMP2 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
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- Application Notes
- Isoelectric Point: 5.2
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.
- Preservative
- Dithiothreitol (DTT), ProClin
- Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- Target
- MMP2 (Matrix Metalloproteinase 2 (MMP2))
- Abstract
- MMP2 Products
- Background
- Alternative Names: CLG4, CLG4A, MMP-II, MONA, TBE-1, PEX, Gelatinase A, 72 kDa Gelatinase, 72 kDa Type IV Collagenase
- Molecular Weight
- 64kDa
- UniProt
- P08253
- Pathways
- Activation of Innate immune Response
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