PDGFA Protein (AA 94-194) (His tag)

Catalog No. ABIN6239819

Product Details

Target: PDGFA (Platelet Derived Growth Factor A (PDGFA))

Biological Activity: Active

Protein Characteristics: AA 94-194

Origin: Mouse

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This PDGFA protein is labelled with His tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Characteristics: Tag location: N-terminal His Tag

Purity: > 95 %

Biological Activity Comment: PDGFA (Platelet-derived growth factor subunit A) is a Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. PDGFA has been described as a chemoattractant for monocytes and proven to be able to induce chemotactic migration of THP-1 cells. Therefore, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of PDGFA on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (100uL cell suspension,106cells/mL in RPMI 1640 with 0.5% FBS) and PDGFA (1.5ng/mL, 7.5ng/mL and 15ng/mL diluted separately in serum free RPMI 1640 ) was added in lower chamber with a polycarbonate filter (8μm pore size) used to separate the two compartments. After incubation at 37oC with 5%CO2 for 3h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (x40) and the number of migrated cells were counted at high magnification (x400) randomly (five fields for each filter). Result shows PDGFA is able to induce migration of THP-1 cells. The migrated THP-1 cells in low chamber at low magnification (x40) were shown in Figure 1. Five fields of each chamber were randomly chosen, and the migrated cells were counted at high magnification (x400). Statistical results were shown in Figure 2. The optimum chemotaxis of PDGFA occurs at 1.5ng/mL.

Application Details

Application Notes: Isoelectric Point: 8.9

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), Other preservative, ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Target Details

Target: PDGFA (Platelet Derived Growth Factor A (PDGFA))

Alternative Name: Platelet Derived Growth Factor Subunit A (PDGFA) (PDGFA Products)

Background: Alternative Names: PDGF-A, PDGF1, Platelet Derived Growth Factor Alpha Polypeptide

Molecular Weight: 17kDa

UniProt: P20033

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling