ARG Protein (AA 1-322) (His tag)
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- Target See all ARG Proteins
- ARG (Arginase (ARG))
- Biological Activity
- Active
- Protein Characteristics
- AA 1-322
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This ARG protein is labelled with His tag.
- Application
- Activity Assay (AcA), Cell Culture (CC)
- Characteristics
- Tag location: N-terminal His Tag
- Purity
- > 97 %
- Biological Activity Comment
- Arginase (Arg) is an enzyme that catalyzes the degradation of arginine to produce urea and omithine, which is crucial in the urea cycle. In most mammals, two isozymes of this enzyme exist; the first, Arginase I, functions in the urea cycle, and is located primarily in the cytoplasm of the liver. The second isozyme, Arginase II, has been implicated in the regulation of the arginine/ornithine concentrations in the cell. Besides, Ubiquitin Carboxyl Terminal Hydrolase L5 (UCHL5) has been identified as an interactor of Arg, thus a binding ELISA assay was conducted to detect the interaction of recombinant human Arg and recombinant human UCHL5. Briefly, Arg were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to UCHL5-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-Arg pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of Arg and UCHL5 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of Arg with UCHL5.
- Top Product
- Discover our top product ARG Protein
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Arginase (ARG) (AA 1-323) (Active) protein (His tag)
ARG Origin: Rat Host: Escherichia coli (E. coli) > 97 % AcA, CC Active
Arginase (ARG) (AA 1-323) (Active) protein (His tag)ARG Origin: Mouse Host: Escherichia coli (E. coli) > 95 % AcA, CC Active
Arginase (ARG) proteinARG Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % SDS, WB, ELISA, IP
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- Application Notes
- Isoelectric Point: 6.7
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- Preservative
- Dithiothreitol (DTT), Other preservative, ProClin
- Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- Target
- ARG (Arginase (ARG))
- Abstract
- ARG Products
- Background
- Alternative Names: ARG1, Arginase I, Liver Arginase
- Molecular Weight
- 37kDa
- UniProt
- P05089
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