ARG Protein (AA 1-323) (His tag)

Catalog No. ABIN6239897

Product Details

Target: ARG (Arginase (ARG))

Biological Activity: Active

Protein Characteristics: AA 1-323

Origin: Rat

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This ARG protein is labelled with His tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Characteristics: Tag location: N-terminal His Tag

Purity: > 97 %

Biological Activity Comment: Arginase (Arg) is an enzyme that catalyzes the degradation of arginine to produce urea and omithine, which is crucial in the urea cycle. In most mammals, two isozymes of this enzyme exist; the first, Arginase I, functions in the urea cycle, and is located primarily in the cytoplasm of the liver. The second isozyme, Arginase II, has been implicated in the regulation of the arginine/ornithine concentrations in the cell. Besides, Estrogen Receptor Alpha (ERa) has been identified as an interactor of, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat Arginase( Arg) and recombinant rat ERa. Briefly, Arg were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ERa-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-Arg pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of Arg and ERa was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of Arg with ERa.

Application Details

Application Notes: Isoelectric Point: 6.7

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), Other preservative, ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Target Details

Target: ARG (Arginase (ARG))

Abstract: ARG Products

Synonyms: CG18104 Protein, Dmel\\CG18104 Protein, EG:171D11.4 Protein, EG:65F1.3 Protein, SI:zC146F4.4 (novel protein with NUDIX domain) Protein, si:ch211-146f4.3 Protein, Tb08.26N11.490 Protein, rocF Protein, NV10213 Protein, argi1 Protein, arginase Protein, arginase 1 L homeolog Protein, arginase 1 Protein, Arginase-1 Protein, arg Protein, arg1.L Protein, arg1 Protein, ARGAH1 Protein, BP0538 Protein, rocF Protein, SAS2066 Protein, RR_RS05730 Protein, CND03500 Protein, CNG00550 Protein, Tb927.8.2020 Protein, PGTG_16455 Protein, LOC100123155 Protein, argi1 Protein

Background: Alternative Names: ARG1, Arginase I, Liver Arginase

Molecular Weight: 37kDa

UniProt: P07824