AATK Protein (GST tag)

Catalog No. ABIN6699529

Supplier Product No.: 010-001-r36s

Product Details

Target: AATK (Apoptosis-Associated tyrosine Kinase (AATK))

Protein Type: Recombinant

Origin: Mouse

Source: Insect cells (Sf9)

Purification tag / Conjugate: This AATK protein is labelled with GST tag.

Application: Western Blotting (WB)

Supplier Product No.: 010-001-r36s

Supplier: Rockland

Purpose: AATK recombinant protein-GST fusion protein

Purification: Recombinant mouse AATK (8-411) was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >95% by densitometry.

Purity: >95%

Application Details

Application Notes:

Western_Blot_Dilution: User Optimized

Application_Note: AATK Protein is suitable for use in Western Blot. Expect a band approximately ~ 71 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 μg/μL

Buffer: AATK Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: AATK (Apoptosis-Associated tyrosine Kinase (AATK))

Alternative Name: Aatk (AATK Products)

Background:

Synonyms: AATYK, LMTK1, KIAA0641, Serine/threonine-protein kinase LMTK1, Apoptosis-associated tyrosine kinase, Brain apoptosis-associated tyrosine kinase, CDK5-binding protein, Lemur tyrosine kinase 1, p35-binding protein, p35BP

Background: AATK or apoptosis-associated tyrosine kinase contains a tyrosine kinase domain at the N-terminus and a proline-rich domain at the C-terminus. AATK is induced during apoptosis, and expression of this protein is a necessary pre-requisite for the induction of growth arrest and/or apoptosis of myeloid precursor cells (1). AATK is highly detected in brain, lung, kidney, and pancreas (2). AATK is also shown to produce neuronal differentiation in a neuroblastoma cell line. AATK Protein is ideal for investigators involved in Signaling Proteins , Cellular Proteins, Apoptosis/Autophagy, Cancer, and Cytoplasmic Tyrosine Kinase research.

NCBI Accession: NM_007377

Pathways: RTK Signaling, Regulation of Cell Size