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ATG13 Protein (GST tag)

ATG13 Origin: Human Host: Escherichia coli (E. coli) Recombinant >70% WB
Rockland
Catalog No. ABIN6699535
Supplier Product No.: 009-001-r44s
  • Target See all ATG13 Proteins
    ATG13 (Autophagy Related 13 (ATG13))
    Protein Type
    Recombinant
    Origin
    • 5
    • 2
    • 1
    • 1
    Human
    Source
    • 2
    • 2
    • 2
    • 2
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This ATG13 protein is labelled with GST tag.
    Application
    Western Blotting (WB)
    Supplier Product No.
    009-001-r44s
    Supplier
    Rockland
    Purpose
    ATG13 recombinant protein-GST fusion protein
    Purification
    Recombinant full-length human ATG13 was expressed by baculovirus in E.coli using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >70% by densitometry.
    Purity
    >70%
    Top Product
    Discover our top product ATG13 Protein
  • Application Notes

    Western_Blot_Dilution: User Optimized

    Application_Note: ATG13 Protein is suitable for use in Western Blot. Expect a band approximately ~ 96 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.1 μg/μL
    Buffer
    ATG13 Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
    Storage
    -80 °C
    Storage Comment
    Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
    Expiry Date
    12 months
  • Target
    ATG13 (Autophagy Related 13 (ATG13))
    Alternative Name
    ATG13 (ATG13 Products)
    Background

    Synonyms: KIAA0652, Autophagy-related protein 13, ATG13

    Background: ATG13 is a protein that is part of the autophagy complex which is the major route by which cytoplasmic contents are delivered to the lysosome for degradation. ATG13 localizes on the autophagic isolation membrane and is essential for autophagosome formation. In mammals, ATG13 interacts with ATG1 (ULK1/2) and FIP200 to form the autophagy. ATG101 can also bind to ATG13 protein and is important for the stability and basal phosphorylation of ATG13 and ULK1 (1). mTOR can suppress the autophagy process through direct regulation of the ULK1-Atg13-FIP200 complex where mTOR has been shown to phosphorylate ATG13 (2). ATG13 Protein is ideal for investigators involved in Signaling Proteins, Apoptosis Proteins, Angiogenesis, Angiogenesis, Apoptosis/Autophagy, Apoptosis/Autophagy, Cardiovascular Disease, Cardiovascular Disease, Cellular Stress, Cellular Stress, Inflammation, Inflammation, Metabolic Disorder, Metabolic Disorder, Neurobiology, Neurobiology, NfkB Pathway, NfkB Pathway, p38 Pathway, and p38 Pathway research.

    NCBI Accession
    NM_001142673
    Pathways
    PI3K-Akt Signaling, Autophagy
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