BCR Protein (GST tag)
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- Target See all BCR Proteins
- BCR (Breakpoint Cluster Region (BCR))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Insect cells (Sf9)
- Purification tag / Conjugate
- This BCR protein is labelled with GST tag.
- Application
- Western Blotting (WB)
- Supplier Product No.
- 009-001-r50s
- Supplier
- Rockland
- Purpose
- BCR recombinant protein-GST fusion protein
- Purification
- Recombinant human BCR (1-695) was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >75% by densitometry.
- Purity
- >75%
- Top Product
- Discover our top product BCR Protein
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Breakpoint Cluster Region (BCR) (AA 1-1271) protein (His tag)
custom-made BCR Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Breakpoint Cluster Region (BCR) (AA 1-1270) protein (His tag)custom-made BCR Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Breakpoint Cluster Region (BCR) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)BCR Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
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Western_Blot_Dilution: User Optimized
Application_Note: BCLR Protein is suitable for use in Western Blot. Expect a band approximately ~ 120 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.2 μg/μL
- Buffer
- BCLR Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
- Expiry Date
- 12 months
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- Target
- BCR (Breakpoint Cluster Region (BCR))
- Alternative Name
- BCR (BCR Products)
- Background
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Synonyms: ALL, CML, PHL, BCR1, D22S11, D22S662, FLJ1645, Breakpoint cluster region protein, Renal carcinoma antigen NY-REN-26, BCR, BCR1, D22S11
Background: BCR (also known as breakpoint cluster region) encodes a phosphoprotein that has serine/threonine kinase activity (1). The human c-ABL oncogene is translocated from chromosome 9 to a region on chromosome 22 (associated with BCR) in chronic myelocytic leukemia. In classical t(9,22) translocation, as observed in chronic granulocytic leukemia (CGL), a hybrid DNA unit is produced comprising the BCR gene product plus the translocated c-ABL gene from chromosome 9. The BCR-ABL hybrid protein (p210) is formed that displays increased tyrosine kinase activity. A similar DNA rearrangement of the p210 protein is also found in cases of Philadelphia-positive acute lymphoblastic leukemia (ALL) (2). BCR Protein is ideal for investigators involved in Signaling Proteins, Cellular Proteins, and Cancer research.
- NCBI Accession
- NM_004327
- Pathways
- Regulation of Leukocyte Mediated Immunity, Platelet-derived growth Factor Receptor Signaling
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