CDKL5 Protein (GST tag)

Catalog No. ABIN6699779

Supplier Product No.: 009-001-t59s

Product Details

Target: CDKL5 (Cyclin-Dependent Kinase-Like 5 (CDKL5))

Protein Type: Recombinant

Origin: Human

Source: Insect cells (Sf9)

Purification tag / Conjugate: This CDKL5 protein is labelled with GST tag.

Application: Western Blotting (WB)

Supplier Product No.: 009-001-t59s

Supplier: Rockland

Purpose: STK9 recombinant protein-GST fusion protein

Purification: Recombinant human STK9 (CDKL5) (1-498) was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.

Purity: >90%

Application Details

Application Notes:

Western_Blot_Dilution: User Optimized

Application_Note: STK9 Protein is suitable for use in Western Blot. Expect a band approximately ~84 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 μg/μL

Buffer: STK9 Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: CDKL5 (Cyclin-Dependent Kinase-Like 5 (CDKL5))

Alternative Name: CDKL5 (CDKL5 Products)

Background:

Synonyms: CDKL5, EIEE2, ISSX, Cyclin-dependent kinase-like 5, STK9

Background: STK9 or CDKL5 is a member of Ser/Thr protein kinase family which has protein kinase activity and is associated with X-linked infantile spasm syndrome (ISSX), also known as X-linked West syndrome, and Rett syndrome (RTT). STK9 can control nuclear speckle morphology by regulating the phosphorylation state of splicing regulatory proteins, and may be involved indirectly in pre-mRNA processing, by controlling splicing factor dynamics (1). The mutations in the STK9 are a major cause of infantile spasms and early epileptic seizures in female patients, and of a later intractable seizure disorder (2). STK9 Protein is ideal for investigators involved in Signaling Proteins, Cellular Proteins, Cardiovascular Disease, ERK/MAPK Pathway, Neurobiology, PKA/PKC Pathway, and Ser/Thr Kinases research.

NCBI Accession: NM_003159

Pathways: Regulation of Cell Size