ELK1 Protein (GST tag)
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- Target See all ELK1 Proteins
- ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This ELK1 protein is labelled with GST tag.
- Application
- Western Blotting (WB)
- Supplier Product No.
- 009-001-r88s
- Supplier
- Rockland
- Purpose
- ELK1 recombinant protein-GST fusion protein
- Purification
- Recombinant full-length human ELK1 was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >75% by densitometry.
- Purity
- >75%
- Top Product
- Discover our top product ELK1 Protein
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- Application Notes
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Western_Blot_Dilution: User Optimized
Other: Kinase Assay-User Optimized
Application_Note: ELK1 Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~ 81 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.2 μg/μL
- Buffer
- ELK1 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
- Expiry Date
- 12 months
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- Target
- ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))
- Alternative Name
- ELK1 (ELK1 Products)
- Background
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Synonyms: ELK1, ETS domain-containing protein Elk-1
Background: ELK1 is a member of the ETS oncogene family of transcription factors (1). ELK1 protein interacts with promoter of the c-fos proto-oncogene and has been shown to be involved in the Ras-Raf-MAPK signaling cascade (1). Immature phosphorylation of transcription factor ELK-1 is implicated in premature aging syndrome and insulin resistance. Expression studies have revealed that elevated expression of ELK1-like protein in human esophageal can result in squamous cell carcinoma (2). ELK1 Protein is ideal for investigators involved in Signaling Proteins, Transcription Proteins, Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, ERK/MAPK Pathway, Invasion/Metastasis, and Neurobiology research.
- NCBI Accession
- NM_005229
- Pathways
- MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling
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