ELK1 Protein (GST tag)

Catalog No. ABIN6699791

Supplier Product No.: 009-001-r88s

Product Details

Target: ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This ELK1 protein is labelled with GST tag.

Application: Western Blotting (WB)

Supplier Product No.: 009-001-r88s

Supplier: Rockland

Purpose: ELK1 recombinant protein-GST fusion protein

Purification: Recombinant full-length human ELK1 was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >75% by densitometry.

Purity: >75%

Application Details

Application Notes:

Western_Blot_Dilution: User Optimized

Other: Kinase Assay-User Optimized

Application_Note: ELK1 Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~ 81 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: ELK1 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))

Alternative Name: ELK1 (ELK1 Products)

Background:

Synonyms: ELK1, ETS domain-containing protein Elk-1

Background: ELK1 is a member of the ETS oncogene family of transcription factors (1). ELK1 protein interacts with promoter of the c-fos proto-oncogene and has been shown to be involved in the Ras-Raf-MAPK signaling cascade (1). Immature phosphorylation of transcription factor ELK-1 is implicated in premature aging syndrome and insulin resistance. Expression studies have revealed that elevated expression of ELK1-like protein in human esophageal can result in squamous cell carcinoma (2). ELK1 Protein is ideal for investigators involved in Signaling Proteins, Transcription Proteins, Angiogenesis, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, ERK/MAPK Pathway, Invasion/Metastasis, and Neurobiology research.

NCBI Accession: NM_005229

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling