EGF Protein

Catalog No. ABIN6699794

Supplier Product No.: 009-001-c26

Product Details

Target: EGF (Epidermal Growth Factor (EGF))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Supplier Product No.: 009-001-c26

Supplier: Rockland

Purpose: EGF Human Recombinant Epidermal Growth Factor

Purification: Purity was determined to be greater than 95% as determined by analysis by RP-HPLC and by reducing and non-reducing SDS-PAGE, against known standard.

Purity: 95,00%

Endotoxin Level: Measured by LAL is 0.5 EU/μg protein.

Biological Activity Comment: The ED50, calculated by the dose-dependent proliferation of murine BALB/c 3T3 cells, is 30-40 pg/ml.

Application Details

Application Notes:

Application Note: EGF Recombinant Protein is suitable as a control for polyclonal or monoclonal anti-EGF in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 5.0 mL

Concentration: 0.1 mg/mL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

References

Khan, Park, Fischer, Dong, Lungjangwa, Jimenez, Casalena, Chew, Dietmann, Auld, Jaenisch, Theunissen: "Probing the signaling requirements for naive human pluripotency by high-throughput chemical screening." in: Cell reports, Vol. 35, Issue 11, pp. 109233, (2022) (PubMed).

Dong, Beltcheva, Gontarz, Zhang, Popli, Fischer, Khan, Park, Yoon, Xing, Kommagani, Wang, Solnica-Krezel, Theunissen: "Derivation of trophoblast stem cells from naïve human pluripotent stem cells." in: eLife, Vol. 9, (2021) (PubMed).

Target Details

Target: EGF (Epidermal Growth Factor (EGF))

Alternative Name: EGF (EGF Products)

Background:

Synonyms: Epidermal growth factor, Urogastrone

Background: EGF (Epidermal Growth Factor) protein, of the superfamily with the same name, is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. It is a magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4 (HOMG4). Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants. This protein exists in the membrane as a single-pass type I membrane protein, and contains 9 EGF-like domains as well as 9 LDL-receptor class B repeats. It is significantly expressed in kidney, salivary gland, cerebrum and prostate.

UniProt: P01133

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation