EGF Protein

Catalog No. ABIN987821

This not_set EGF protein is produced in Escherichia coli (E. coli). There is 1 publication available.

Quick Overview for EGF Protein (ABIN987821)

Target: EGF (Epidermal Growth Factor (EGF))

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by (a) Analysis by SEC-HPLC (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel

Product Details

Sequence: NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW EL

Characteristics: The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells is less then 2 ng/ml, corresponding to a specific activity of 5.0×105 IU/ mg.

Endotoxin Level: Level Less than 0.1ng/myg (1 IEU/myg) determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is recommended to reconstitute the lyophilized Recombinant Human Epidermal Growth Factor (EGF) in sterile 18 Mohm-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. Quantitation Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 2.858 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).2. RP-HPLC analysis, using a calibrated solution of EGF as a reference standard.

Storage: -20 °C

References

Li, Niu, Lang, Guo, Ma, Kiesewetter, Backer, Shen, Chen: "PET imaging of EGF receptors using [18F]FBEM-EGF in a head and neck squamous cell carcinoma model." in: European journal of nuclear medicine and molecular imaging, Vol. 39, Issue 2, pp. 300-8, (2012) (PubMed).

Target Details

Target: EGF (Epidermal Growth Factor (EGF))

Background: Human Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells. Human Epidermal Growth Factor (EGF) is a 6,200 Da protein containing 53 amino acid residues. Synonym: rHuEGF, Epidermal Growth Facto. Formulation: The protein was lyophilized after extensive dialysis against 10mM Phosphate buffer, pH7.0, 200mM NaCl buffer.

Molecular Weight: 6.0 kDa+/-10% determined by reduced SDS-PAGE Isoelectric Point The main zone between 4.0-5.0 analysis by IEF UV Scan The maximal absorption wave is 275+/-3 nm.

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation