NFATC1 Protein (GST tag)

Catalog No. ABIN6700318

Supplier Product No.: 009-001-s56s

Product Details

Target: NFATC1 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1))

Protein Type: Recombinant

Origin: Human

Source: Insect cells (Sf9)

Purification tag / Conjugate: This NFATC1 protein is labelled with GST tag.

Application: Western Blotting (WB)

Supplier Product No.: 009-001-s56s

Supplier: Rockland

Purpose: NFATC1 recombinant protein-GST fusion protein

Purification: Recombinant full-length human NFATC1 was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.

Purity: >90%

Application Details

Application Notes:

Western_Blot_Dilution: User Optimized

Application_Note: NFATC1 Protein is suitable for use in Western Blot. Expect a band approximately ~125 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: NFATC1 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: NFATC1 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1))

Alternative Name: NFATC1 (NFATC1 Products)

Background:

Synonyms: NF-ATC, NFATc, NFAT2, MGC138448, Nuclear factor of activated T-cells, cytoplasmic 1 NF-ATc1, NFATc1, NFAT transcription complex cytosolic component

Background: NFATC1 is a member of the NFAT family of proteins which are Ca2+/calcineurin-responsive transcription factors primarily recognized for their central roles in T lymphocyte activation and cardiac valve development (1). NFAT consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T-cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to the NFAT family of transcription factors play a central role in inducible gene transcription during immune response. The product of NFATC1 is an inducible nuclear component and functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A (2). NFATC1 Protein is ideal for investigators involved in Signaling Proteins, Transcription Proteins, Cardiovascular Disease, Inflammation, JNK/SAPK Pathway, NfkB Pathway, and p38 Pathway research.

NCBI Accession: NM_172390

Pathways: RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway