PDHA1 Protein (His tag)

Catalog No. ABIN6700438

Supplier Product No.: 009-001-s86

Product Details

Target: PDHA1 (Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This PDHA1 protein is labelled with His tag.

Application: Western Blotting (WB)

Supplier Product No.: 009-001-s86

Supplier: Rockland

Purpose: PDHA1 recombinant protein Substrate-HIS Epitope

Purification: Recombinant human PDHA1 (30-390) was expressed in E. coli cells using an N-Terminal his epitope. The purity was determined to be >85% by densitometry.

Purity: >85%

Application Details

Application Notes:

Western_Blot_Dilution: User Optimized

Other: Kinase Assay-User Optimized

Application_Note: PDHA1 Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~47 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: PDHA1 Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: PDHA1 (Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1))

Alternative Name: PDHA1 (PDHA1 Products)

Background:

Synonyms: PDHA, PDHCE1A, PHE1A, Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial, PDHE1-A type I, PDHA1, PHE1A

Background: PDHA1 pyruvate dehydrogenase (lipoamide) alpha 1 is a member of the pyruvate dehydrogenase (PDH) complex. PDHA1 is a nuclear-encoded mitochondrial multienzyme complex which catalyzes the overall conversion of pyruvate to acetyl-CoA and CO (2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle . PDHA1 plays a critical role in the brain that usually obtains all of its energy from the aerobic oxidation of glucose (1). Mutations in the PDHA1 are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome (2). PDHA1 Protein is ideal for investigators involved in Signaling Reagents, Protein Substrates, Apoptosis/Autophagy, Inflammation, Metabolic Disorder, and Ser/Thr Kinases research.

NCBI Accession: NM_000284

Pathways: Warburg Effect