VHL Protein (AA 1-172) (GST tag)

Catalog No. ABIN1325000

Product Details

Target: VHL (Von Hippel-Lindau Tumor Suppressor, E3 Ubiquitin Protein Ligase (VHL))

Protein Type: Recombinant

Protein Characteristics: AA 1-172

Origin: Human

Source: Wheat germ

Purification tag / Conjugate: This VHL protein is labelled with GST tag.

Application: Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)

Purpose: VHL (Human) Recombinant Protein (P01)

Sequence: MPRRAENWDE AEVGAEEAGV EEYGPEEDGG EESGAEESGP EESGPEELGA EEEMEAGRPR PVLRSVNSRE PSQVIFCNRS PRVVLPVWLN FDGEPQPYPT LPPGTGRRIH SYRVYTLKER CLQVVRSLVK PENYRRLDIV RSLYEDLEDH PNVQKDLERL TQERIAHQRM GD

Characteristics: Human VHL full-length ORF ( ENSP00000344757, 1 a.a. - 172 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Kim, Lee, Jang, Yi, Kim, Han, Lee, Tong, Vincelette, Gao, Yin, Evans, Choi, Qin, Liu, Zhang, Deng, Jen, Zhang, Wang, Lou: "WSB1 promotes tumor metastasis by inducing pVHL degradation." in: Genes & development, Vol. 29, Issue 21, pp. 2244-57, (2015) (PubMed).

Target Details

Target: VHL (Von Hippel-Lindau Tumor Suppressor, E3 Ubiquitin Protein Ligase (VHL))

Alternative Name: VHL (VHL Products)

Background: Full Gene Name: von Hippel-Lindau tumor suppressor
Synonyms: HRCA1,RCA1,VHL1

Gene ID: 7428

Pathways: Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Ubiquitin Proteasome Pathway