NLRP3 Protein (AA 2-1036) (DYKDDDDK Tag)
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- Target See all NLRP3 Proteins
- NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-1036
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This NLRP3 protein is labelled with DYKDDDDK Tag.
- Purpose
- Recombinant Human NLRP3 with C-terminal Flag tag
- Specificity
- NLRP3 (Lys2-Trp1036) Flag
- Characteristics
- Extracellular Domain Protein
- Purification
- Purified from cell culture supernatant by affinity chromatography
- Purity
- The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
- Top Product
- Discover our top product NLRP3 Protein
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NLR Family, Pyrin Domain Containing 3 (NLRP3) (AA 1-1036) protein (His tag)
custom-made NLRP3 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
NLR Family, Pyrin Domain Containing 3 (NLRP3) (AA 1-1033) protein (His tag)custom-made NLRP3 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
NLR Family, Pyrin Domain Containing 3 (NLRP3) (AA 1-1036) protein (GST tag)NLRP3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
NLR Family, Pyrin Domain Containing 3 (NLRP3) (AA 5-218) protein (His-IF2DI Tag)NLRP3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
NLR Family, Pyrin Domain Containing 3 (NLRP3) (AA 1-100) protein (GST tag)NLRP3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- Expiry Date
- 12 months
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- Target
- NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))
- Alternative Name
- NLRP3 (NLRP3 Products)
- Background
- This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID). Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data, however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid. [provided by RefSeq, Oct 2008]
- Molecular Weight
- predicted molecular mass of 121.8 kDa after removal of the signal peptide.
- UniProt
- Q96P20
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Inflammasome
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