Claudin 18.2 (Active) protein (His tag,Twin-Strep tag)

Catalog No. ABIN7199139

Product Details

Target: Claudin 18.2

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: His tag,Twin-Strep tag

Purpose: Human Claudin-18.2 Protein, His,Twin-Strep Tag (active membrane protein)

Sequence: Met 1 - Ala 200

Characteristics: Human Claudin-18.2, His,Twin-Strep Tag (CL2-H5587) is expressed from Baculovirus-Insect cells. It contains AA Met 1 - Ala 200 (Accession # P56856-2).

Purity: >80 % as determined by SDS-PAGE.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Application Notes: This protein carries a polyhistidine tag at the N-terminus and a twin strep tag at the C-terminus. The protein has a calculated MW of 26.3 kDa. The protein migrates as 20 kDa and 40 kDa under non-reducing (NR) condition due to glycosylation.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH 7.5

Storage: -80 °C

Storage Comment: -70°C

Target Details

Target: Claudin 18.2

Alternative Name:  Claudin-18.2 

Background: Synonyms: Claudin-18.2,CLDN18,Claudin-18,
Description: Claudins (CLDNs) are a family of proteins that form tight junctions and maintain the polarity of epithelial and endothelial cells. CLDN18 is specifically expressed in the stomach and lung. Of the two CLDN18 isoform transcripts produced by alternative splicing, CLDN18.2 is a highly selective gastric lineage marker that determines the gastric phenotype in a neoplastic condition, whereas CLDN18.1 is lung specific. CLDN18.2 is a highly selective gastric lineage antigen expressed exclusively on short-lived differentiated gastric epithelial cells where it has only limited accessibility to antibody drugs.14,15 CLDN18.2 is maintained during the course of malignant transformation and thus frequently displayed on the surface of human gastric cancer cells.

Molecular Weight: 26.3 kDa

NCBI Accession: NP_001002026