BACE1 Protein (AA 22-457) (His tag)

Catalog No. ABIN7273904

Product Details

Target: BACE1 (Beta-secretase 1 (BACE1))

Protein Type: Recombinant

Protein Characteristics: AA 22-457

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This BACE1 protein is labelled with His tag.

Purpose: Human BACE-1 Protein

Sequence: Thr22-Thr457

Characteristics: Recombinant Human BACE-1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr22-Thr457.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: BACE1 (Beta-secretase 1 (BACE1))

Alternative Name: BACE-1 (BACE1 Products)

Background: The beta-site amyloid precursor protein cleaving enzyme-1 (BACE-1) initiates the generation of amyloid-β (Aβ), and the amyloid cascade leading to amyloid plaque deposition, neurodegeneration, and dementia in Alzheimer's disease (AD). Clinical failures of anti-Aβ therapies in dementia stages suggest that treatment has to start in the early, asymptomatic disease states.

Molecular Weight: 49.5 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.

NCBI Accession: NP_036236