CD3 epsilon Protein (CD3E) (AA 23-126) (Fc Tag)
-
- Target See all CD3 epsilon (CD3E) Proteins
- CD3 epsilon (CD3E)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 23-126
-
Origin
- Human
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CD3 epsilon protein is labelled with Fc Tag.
- Purpose
- Human CD3E/CD3 epsilon Protein
- Sequence
- Asp23-Asp126 (C119S, C122S)
- Characteristics
- Recombinant Human CD3E/CD3 epsilon Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Asp23-Asp126(C119S, C122S).
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human CD3E at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, mFc Tag with the EC50 of 26.7ng/ml determined by ELISA. See testing image for detail.
- Top Product
- Discover our top product CD3E Protein
-
CD3 epsilon (CD3E) protein (Fc Tag)
CD3E Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE
CD3 epsilon (CD3E) protein (His tag)CD3E Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
CD3 epsilon (CD3E) (AA 23-48) protein (Fc-Avi Tag)CD3E Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3 epsilon (CD3E) protein (His tag)CD3E Origin: Human Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE
CD3 epsilon (CD3E) (AA 22-48) protein (Fc-Avi Tag)CD3E Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3 epsilon (CD3E) (AA 23-48) protein (Fc-Avi Tag,Biotin)CD3E Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3 epsilon (CD3E) (AA 22-48) protein (Fc-Avi Tag,Biotin)CD3E Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3 epsilon (CD3E) (Active) protein (mFc Tag)CD3E Origin: Human Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE. Active
CD3 epsilon (CD3E) (AA 23-126) protein (His tag,AVI tag,Biotin)CD3E Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE.
CD3 epsilon (CD3E) (AA 22-117) protein (Fc Tag)CD3E Origin: Cynomolgus Host: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
-
- Target
- CD3 epsilon (CD3E)
- Alternative Name
- CD3E (CD3E Products)
- Background
- T-cell surface glycoprotein CD3 epsilon & CD3 gamma chain, also known as CD3E & CD3G , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
- Molecular Weight
- 36.1 kDa. Due to glycosylation, the protein migrates to 35-55 kDa based on Tris-Bis PAGE result.
- UniProt
- P07766
- Pathways
- TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
-
