CD3 epsilon Protein (CD3E) (Fc Tag)
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- Target See all CD3 epsilon (CD3E) Proteins
- CD3 epsilon (CD3E)
- Protein Type
- Recombinant
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Origin
- Cynomolgus
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CD3 epsilon protein is labelled with Fc Tag.
- Sequence
- Gln22-Asp117
- Purity
- > 95% as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Cynomolgus CD3E, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD3E Antibody, mFc Tag with the EC50 of 39.4ng/ml determined by ELISA. See testing image for detail.
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CD3 epsilon (CD3E) protein (His tag)
CD3E Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
CD3 epsilon (CD3E) (AA 23-48) protein (Fc-Avi Tag)CD3E Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3 epsilon (CD3E) protein (His tag)CD3E Origin: Human Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE
CD3 epsilon (CD3E) (AA 23-126) protein (Fc Tag)CD3E Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
CD3 epsilon (CD3E) (AA 22-48) protein (Fc-Avi Tag)CD3E Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3 epsilon (CD3E) (AA 23-48) protein (Fc-Avi Tag,Biotin)CD3E Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3 epsilon (CD3E) (AA 22-48) protein (Fc-Avi Tag,Biotin)CD3E Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3 epsilon (CD3E) (Active) protein (mFc Tag)CD3E Origin: Human Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE. Active
CD3 epsilon (CD3E) (AA 23-126) protein (His tag,AVI tag,Biotin)CD3E Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE.
CD3 epsilon (CD3E) (AA 23-126) protein (His tag)CD3E Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- CD3 epsilon (CD3E)
- Alternative Name
- CD3E (CD3E Products)
- Synonyms
- cd3e Protein, CD3E Protein, t3e Protein, tcre Protein, AI504783 Protein, CD3 Protein, CD3epsilon Protein, T3e Protein, T3E Protein, TCRE Protein, CD3e molecule Protein, CD3 antigen, epsilon polypeptide Protein, cd3e Protein, CD3E Protein, Cd3e Protein
- Background
- CD3e, CD3E, T3E, FLJ18683, TCRE, CD3-epsilon, IMD18,CD3E, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.
- Molecular Weight
- 36.9 kDa. Due to glycosylation, the protein migrates to 43-48 kDa based on Tris-Bis PAGE result.
- Pathways
- TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
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