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CD33 Protein (CD33) (AA 18-259) (His-Avi Tag)

CD33 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE, > 95 % as determined by HPLC
Catalog No. ABIN7274159
  • Target See all CD33 Proteins
    CD33
    Protein Type
    Recombinant
    Protein Characteristics
    AA 18-259
    Origin
    • 33
    • 4
    • 1
    • 1
    Human
    Source
    • 28
    • 4
    • 3
    • 2
    • 1
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This CD33 protein is labelled with His-Avi Tag.
    Purpose
    Human Siglec-3/CD33 Protein
    Sequence
    Asp18-His259
    Characteristics
    Recombinant Human Siglec-3/CD33 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus., It contains Asp18-His259.
    Purity
    > 95 % as determined by Tris-Bis PAGE, > 95 % as determined by HPLC
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Less than 1 EU per μg by the LAL method.
    Biological Activity Comment
    Immobilized Human Siglec-3, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA. See testing image for detail.
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in 20 mM MES, 500 mM NaCl, 200 mM L-Arginine ( pH 5.0).
    Buffer
    Lyophilized from 0.22 μm filtered solution in 20 mM MES, 500 mM NaCl, 200 mM L-Arginine ( pH 5.0). Normally 8 % mannitol is added as protectant before lyophilization.
    Storage
    -20 °C,-80 °C
    Storage Comment
    -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    CD33
    Alternative Name
    Siglec-3 (CD33 Products)
    Background
    Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state.They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglecs 5 to 11. To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs.
    Molecular Weight
    29.6 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.
    UniProt
    P20138-1
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