CD33 Protein (CD33) (AA 18-259) (Fc Tag)
Quick Overview for CD33 Protein (CD33) (AA 18-259) (Fc Tag) (ABIN7275588)
Target
See all CD33 ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 18-259
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Purification tag / Conjugate
- This CD33 protein is labelled with Fc Tag.
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Purpose
- Human Siglec-3/CD33 Protein
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Sequence
- Asp18-His259
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Characteristics
- Recombinant Human Siglec-3/CD33 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Asp18-His259.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human Siglec-3 at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 33.9ng/ml determined by ELISA. See testing image for detail.
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CD33 (CD33) (AA 18-259) protein (His-Avi Tag)
CD33 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE, > 95 % as determined by HPLC
CD33 (CD33) (AA 18-259) protein (His-Avi Tag,Biotin)CD33 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
CD33 (CD33) (AA 17-248) protein (His tag)CD33 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD33 (CD33) (AA 18-259) protein (Fc-His Tag)CD33 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
CD33 (CD33) protein (His tag)CD33 Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
CD33 (CD33) (AA 1-364) protein (GST tag)CD33 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
CD33 (CD33) (AA 18-259) (Active) protein (His-Fc tag)CD33 Origin: Human Host: Mammalian Cells Recombinant >90 % as determined by SDS-PAGE. ELISA, WB, SDS, AcA, Imm Active
CD33 (CD33) (AA 140-259) protein (Fc Tag)CD33 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
CD33 (CD33) (AA 18-240) protein (Fc Tag)CD33 Origin: Mouse Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
CD33 (CD33) (AA 18-259) protein (His tag)CD33 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- CD33
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Alternative Name
- Siglec-3
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Background
- Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state.They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglecs 5 to 11. To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs.
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Molecular Weight
- 53.6 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Tris-Bis PAGE result.
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UniProt
- P20138-1
Target
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