CD58 Protein (CD58) (AA 29-215) (Fc Tag)
-
- Target See all CD58 Proteins
- CD58
- Protein Type
- Recombinant
- Protein Characteristics
- AA 29-215
- Origin
- Human
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CD58 protein is labelled with Fc Tag.
- Purpose
- Human CD58 Protein
- Sequence
- Phe29-Arg215
- Characteristics
- Recombinant Human CD58 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Phe29-Arg215.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- The affinity constant of 9.91 nM as determined in SPR assay (Biacore T200). See testing image for detail.
-
CD58 (CD58) (AA 1-240) protein (GST tag)
CD58 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
CD58 (CD58) (AA 29-215) protein (His tag)CD58 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
CD58 (CD58) (AA 29-215) (Active) protein (Fc Tag)CD58 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
-
- Target
- CD58
- Alternative Name
- CD58 (CD58 Products)
- Synonyms
- LFA-3 Protein, LFA3 Protein, ag3 Protein, CD58 molecule Protein, CD58 Protein
- Background
- Loss of CD58 is a common mechanism for tumor immune evasion in lymphoid malignancies. CD58 loss is known to occur due to both genetic and non-genetic causes, therefore, we hypothesized that restoring CD58 expression in lymphoma cells may be an effective treatment approach. EZH2 is involved in the epigenetic silencing of CD58 in lymphoma cells as a mechanism for tumor immune escape, and EZH2 inhibitors are able to restore epigenetically suppressed CD58 expression.
- Molecular Weight
- 48.1 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result.
-
