CD58 Protein (CD58) (AA 29-215) (His tag)
Quick Overview for CD58 Protein (CD58) (AA 29-215) (His tag) (ABIN5855029)
Target
See all CD58 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 29-215
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Purification tag / Conjugate
- This CD58 protein is labelled with His tag.
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Sequence
- ADPFSQQIYG VVYGNVTFHV PSNVPLKEVL WKKQKDKVAE LENSEFRAFS SFKNRVYLDT VSGSLTIYNL TSSDEDEYEM ESPNITDTMK FFLYVLESLP SPTLTCALTN GSIEVQCMIP EHYNSHRGLI MYSWDCPMEQ CKRNSTSIYF KMENDLPQKI QCTLSNPLFN TTSSIILTTC IPSSGHSRHR HHHHHH
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Purification
- > 95% by SDS-PAGE
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Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
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CD58 (CD58) (AA 1-240) protein (GST tag)
CD58 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
CD58 (CD58) (AA 29-215) protein (His tag)CD58 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
CD58 (CD58) (AA 29-215) (Active) protein (Fc Tag)CD58 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- Phosphate-Buffered Saline ( pH 7.4) containing 10 % glycerol
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- CD58
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Alternative Name
- CD58/LFA-3
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Background
- CD58, also known as lymphocyte function-associated antigen 3 isoform 1, is a cell adhesion molecule expressed on Antigen Presenting Cells (APC), particularly macrophages. It binds to CD2 (LFA-2) on T cells and is important in strengthening the adhesion between the T cells and Professional Antigen Presenting Cells. It is an important regulator of immune cell function which occurs as both surface-associated and soluble forms. It is associated with increased risk for multiple sclerosis. Recombinant human CD58, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
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Molecular Weight
- 22.5 kDa (196aa)
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NCBI Accession
- NP_001770
Target
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