CD40 Ligand Protein (CD40LG) (AA 113-261, Trimer) (His-DYKDDDDK Tag,Biotin)

Catalog No. ABIN7274319

Product Details

Target: CD40 Ligand (CD40LG)

Protein Type: Recombinant

Protein Characteristics: AA 113-261, Trimer

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CD40 Ligand protein is labelled with His-DYKDDDDK Tag,Biotin.

Purpose: Biotinylated Human CD40 Ligand/TNFSF5 Trimer Protein (Primary Amine Labeling)

Sequence: Met113-Leu261

Characteristics: Recombinant Biotinylated Human CD40 Ligand/TNFSF5 Trimer Protein (Primary Amine Labeling) is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Met113-Leu261.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human CD40 Ligand Trimer, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human CD40, hFc Tag with the EC50 of 0.77μg/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD40 Ligand (CD40LG)

Alternative Name: CD40 Ligand (CD40LG Products)

Background: CD40 ligand or CD40L, also called CD154,  is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells.

Molecular Weight: 52.4 kDa, Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result.

UniProt: P29965

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints