CD40 Ligand Protein (CD40LG) (AA 116-261) (His-DYKDDDDK-Strep II Tag)
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- Target See all CD40 Ligand (CD40LG) Proteins
- CD40 Ligand (CD40LG)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 116-261
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Origin
- Human
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Source
- HEK-293T Cells
- Purification tag / Conjugate
- This CD40 Ligand protein is labelled with His-DYKDDDDK-Strep II Tag.
- Application
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Protein Interaction (PI)
- Purpose
- CD40LG (Human) Recombinant Protein
- Sequence
- GDQNPQIAAH VISEASSKTT SVLQWAEKGY YTMSNNLVTL ENGKQLTVKR QGLYYIYAQV TFCSNREASS QAPFIASLCL KSPGRFERIL LRAANTHSSA KPCGQQSIHL GGVFELQPGA SVFVNVTDPS QVSHGTGFTS FGLLKL
- Characteristics
- Purified CD40LG (AAH74950.1, 116 a.a. - 261 a.a.) human recombinant protein with His-Flag-StrepII tag at N-terminus expressed in human cells.
- Purification
- Transfection of pSuper-CD40LG plasmid into HEK293T cell, and the expressed protein was purified by Strep-Tactin affinity column.
- Top Product
- Discover our top product CD40LG Protein
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CD40 Ligand (CD40LG) (Trimer) protein (Fc Tag)
CD40LG Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD40 Ligand (CD40LG) (Trimer) protein (His-DYKDDDDK Tag,Biotin)CD40LG Origin: Human Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
CD40 Ligand (CD40LG) (Trimer) protein (His-DYKDDDDK Tag)CD40LG Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD40 Ligand (CD40LG) (Trimer) protein (Fc Tag)CD40LG Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
CD40 Ligand (CD40LG) (AA 113-261) (Active) protein (Fc Tag)CD40LG Origin: Human, Rhesus Monkey Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD40 Ligand (CD40LG) (AA 116-261) (Active) protein (DYKDDDDK Tag,His tag)CD40LG Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
CD40 Ligand (CD40LG) (Active) protein (His tag)CD40LG Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by reducing SDS-PAGE. Active
CD40 Ligand (CD40LG) proteinCD40LG Origin: Human Host: Human Cells Recombinant >90 % as determined by reducing SDS-PAGE.
CD40 Ligand (CD40LG) (AA 112-260) protein (His tag)CD40LG Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
CD40 Ligand (CD40LG) (AA 1-261) protein (GST tag)CD40LG Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Transfection of pSuper-CD40LG plasmid into HEK293T cell, and the expressed protein was purified by Strep-Tactin affinity column.
Product Quality tested by: SDS-PAGE and Western Blot - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 10 μg/mL
- Buffer
- 100 mM Tris-HCl pH 8.0, 150 mM NaCl, 1 mM EDTA, and 5 mM desthiobiotin.
- Preservative
- Other preservative
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
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- Target
- CD40 Ligand (CD40LG)
- Alternative Name
- CD40LG (CD40LG Products)
- Synonyms
- CD154 Protein, cd40l Protein, CD40L Protein, HIGM1 Protein, IGM Protein, IMD3 Protein, T-BAM Protein, TNFSF5 Protein, TRAP Protein, gp39 Protein, hCD40L Protein, CD40-L Protein, Cd40l Protein, Ly-62 Protein, Ly62 Protein, Tnfsf5 Protein, CD40 ligand Protein, TNF superfamily member 5 Protein, CD40LG Protein, cd40l Protein, Cd40lg Protein
- Background
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Full Gene Name: CD40 ligand
Synonyms: CD154,CD40L,HIGM1,IGM,IMD3,T-BAM,TNFSF5,TRAP,gp39,hCD40L - Gene ID
- 959
- Pathways
- NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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