CSF1R Protein (His-Avi Tag,Biotin)

Catalog No. ABIN7274428

Product Details

Target: CSF1R (Colony Stimulating Factor 1 Receptor (CSF1R))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CSF1R protein is labelled with His-Avi Tag,Biotin.

Sequence: Ile20-Glu512

Purity: > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Anti-CSF R Antibody, hFc Tag at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human M-CSF R, His Tag with the EC50 of 45.4ng/ml determined by ELISA. The affinity constant of 0.81 μM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Storage: 4 °C,-80 °C

Storage Comment: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CSF1R (Colony Stimulating Factor 1 Receptor (CSF1R))

Alternative Name: M-CSF R (CSF1R Products)

Synonyms: AI323359 Protein, CD115 Protein, CSF-1R Protein, Csfmr Protein, Fim-2 Protein, Fms Protein, M-CSF-R Protein, M-CSFR Protein, C-FMS Protein, CSFR Protein, FIM2 Protein, FMS Protein, HDLS Protein, PDGFRB Protein, MGC89146 Protein, CSF-1-R Protein, c-fms Protein, mrfms Protein, CSF1 Protein, M-CSF Protein, csf1r Protein, fms Protein, panther Protein, colony stimulating factor 1 receptor Protein, macrophage colony-stimulating factor receptor Protein, colony stimulating factor 1 receptor, a Protein, Csf1r Protein, CSF1R Protein, m-csfr Protein, csf1r Protein, csf1ra Protein

Background: CD115, CSF1R, CSF-1-R, CSFR, FMSFIM2, M-CSF R, MCSFR, M-CSFR, C-FMS, FIM2, FMS, HDLS,CSF1R also known as M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region.CSF1R is tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes.

Molecular Weight: 57.4 kDa. Due to glycosylation, the protein migrates to 75-105 kDa based on Tris-Bis PAGE result.

Pathways: RTK Signaling, Inositol Metabolic Process, Cell-Cell Junction Organization