CSF2RA Protein (His-Avi Tag,Biotin)

Catalog No. ABIN7274429

Product Details

Target: CSF2RA (Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage) (CSF2RA))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CSF2RA protein is labelled with His-Avi Tag,Biotin.

Sequence: Glu23-Gly320

Purity: > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Anti-GM-CSF R alpha Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GM-CSF R alpha, His Tag with the EC50 of 11.3ng/ml determined by ELISA. The affinity constant of 1.68 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Storage: 4 °C,-80 °C

Storage Comment: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CSF2RA (Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage) (CSF2RA))

Alternative Name: GM-CSF R alpha (CSF2RA Products)

Synonyms: CD116 Protein, CDw116 Protein, CSF2R Protein, CSF2RAX Protein, CSF2RAY Protein, CSF2RX Protein, CSF2RY Protein, GM-CSF-R-alpha Protein, GMCSFR Protein, GMR Protein, SMDP4 Protein, Csfgmra Protein, GM-CSF-Ra Protein, GM-CSFR Protein, colony stimulating factor 2 receptor, alpha Protein, colony stimulating factor 2 receptor alpha subunit Protein, colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage) Protein, CSF2RA Protein, Csf2ra Protein

Background: GM-CSF-R-alpha, GMCSFR-alpha, GMR-alpha, CSF2R, CSF2RY, CSF2RA, CDw116, CD116, CSF2RAX, CSF2RAY, CSF2RX, GMCSFR, GMR, SMDP4,In the first trimester, the GM-CSF-R was found to be present on villous cytotrophoblast and all populations of extravillous trophoblast. Expression by villous syncytiotrophoblast was weak or absent, but this increased markedly by term. GM-CSF-R were also expressed by fetal Hofbauer cells within the mesenchyme of the chorionic villi and by uterine glandular epithelium and decidual macrophages within maternal decidua.

Molecular Weight: 37.7 kDa. Due to glycosylation, the protein migrates to 68-75 kDa based on Tris-Bis PAGE result.

Pathways: JAK-STAT Signaling