IGF1R Protein (AA 31-932) (His-Avi Tag)
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- Target See all IGF1R Proteins
- IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 31-932
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This IGF1R protein is labelled with His-Avi Tag.
- Purpose
- Human IGF1R/CD221 Protein
- Sequence
- Glu31-Asn932
- Characteristics
- Recombinant Human IGF1R/CD221 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu31-Asn932.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human IGF1R, His Tag at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-IGF1R Antibody, hFc Tag with the EC50 of 54.1ng/ml determined by ELISA. See testing image for detail.
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Insulin-Like Growth Factor 1 Receptor (IGF1R) (AA 31-932) protein (His-Avi Tag,Biotin)
IGF1R Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Insulin-Like Growth Factor 1 Receptor (IGF1R) (AA 31-932) (Active) protein (His tag)IGF1R Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Insulin-Like Growth Factor 1 Receptor (IGF1R) (AA 741-935) protein (His-IF2DI Tag)IGF1R Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Insulin-Like Growth Factor 1 Receptor (IGF1R) (AA 31-932) protein (His tag)IGF1R Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
Insulin-Like Growth Factor 1 Receptor (IGF1R) (AA 741-935) protein (His-DYKDDDDK-Strep II Tag)IGF1R Origin: Human Host: HEK-293T Cells Recombinant SDS, WB, ELISA, PI
Insulin-Like Growth Factor 1 Receptor (IGF1R) (AA 31-932) protein (His tag)IGF1R Origin: Cynomolgus Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE.
Insulin-Like Growth Factor 1 Receptor (IGF1R) (AA 31-936) protein (His tag)IGF1R Origin: Rat Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))
- Alternative Name
- IGF1R (IGF1R Products)
- Background
- The type 1 IGF receptor (IGF1R) is a transmembrane tyrosine kinase that is frequently overexpressed by tumours, and mediates proliferation and apoptosis protection. IGF signalling also influences hypoxia signalling, protease secretion, tumour cell motility and adhesion, and thus can affect the propensity for invasion and metastasis. Therefore, the IGF1R is now an attractive anti-cancer treatment target.
- Molecular Weight
- 105.8 kDa (alpha subunit) and 23 kDa (beta subunit). Due to glycosylation, the protein migrates to 110-120 kDa(alpha subunit) and 52-55 kDa(beta subunit) based on Tris-Bis PAGE result.
- UniProt
- P08069
- Pathways
- RTK Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagy
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