IL3RA Protein (AA 19-305) (His-Avi Tag,Biotin)

Catalog No. ABIN7274944

Product Details

Target: IL3RA (Interleukin 3 Receptor, alpha (IL3RA))

Protein Type: Recombinant

Protein Characteristics: AA 19-305

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This IL3RA protein is labelled with His-Avi Tag,Biotin.

Purpose: Biotinylated Human IL-3 R alpha/CD123 Protein

Sequence: Thr19-Arg305

Characteristics: Recombinant Biotinylated Human IL-3 R alpha/CD123 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Thr19-Arg305.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Anti-IL-3 R alpha Antibody at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-3 R alpha, His Tag with the EC50 of 23.0ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL3RA (Interleukin 3 Receptor, alpha (IL3RA))

Alternative Name: IL-3 R alpha (IL3RA Products)

Background: Interleukin-3 receptor subunit alpha, also known as IL-3 receptor subunit alpha, IL-3R-alpha, CD123, and IL3RA, is a single-pass type I membrane protein which belongs to the type I cytokine receptor family and Type 5 subfamily.The specific alpha subunit of the interleukin-3 receptor (IL-3Ralpha, CD123) is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias.

Molecular Weight: 35.99 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.

Pathways: JAK-STAT Signaling