IL18R1 Protein (AA 22-329) (His tag)

Catalog No. ABIN7274971

Product Details

Target: IL18R1 (Interleukin 18 Receptor 1 (IL18R1))

Protein Type: Recombinant

Protein Characteristics: AA 22-329

Origin: Cynomolgus

Source: HEK-293 Cells

Purification tag / Conjugate: This IL18R1 protein is labelled with His tag.

Purpose: Cynomolgus IL-18 R1/CD218a Protein

Sequence: Cys22-Gly329

Characteristics: Recombinant Cynomolgus IL-18 R1/CD218a Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Cys22-Gly329.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: The affinity constant of 2.02 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL18R1 (Interleukin 18 Receptor 1 (IL18R1))

Alternative Name: IL-18 R1 (IL18R1 Products)

Background: Interleukin-18 (IL-18) is a member of the interleukin-1 family of cytokines produced constitutively by different cell types and by adipose tissue. Interleukin (IL)-18 was originally discovered as a factor that enhanced IFN-γ production from anti-CD3-stimulated Th1 cells, especially in the presence of IL-12. Upon stimulation with Ag plus IL-12, naive T cells develop into IL-18 receptor (IL-18R) expressing Th1 cells, which increase IFN-γ production in response to IL-18 stimulation.

Molecular Weight: 36.45 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.

UniProt: A0A2K5V3J9