IL31RA Protein (AA 20-519) (His tag)

Catalog No. ABIN7275018

Product Details

Target: IL31RA (Interleukin 31 Receptor A (IL31RA))

Protein Type: Recombinant

Protein Characteristics: AA 20-519

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This IL31RA protein is labelled with His tag.

Purpose: Human IL-31 RA Protein

Sequence: Ala20-Glu519

Characteristics: Recombinant Human IL-31 RA Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala20-Glu519.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human IL-31 RA, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-31 RA Antibody, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA. The affinity constant of 82.4nM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL31RA (Interleukin 31 Receptor A (IL31RA))

Alternative Name: IL-31 RA (IL31RA Products)

Background: Interleukin-31 receptor A (IL-31RA) is a newly identified type I cytokine receptor, that is related to gp130, the common receptor of the interleukin (IL) -6 family cytokines. IL-31RA forms a functional receptor complex for IL-31 together with the beta subunit of oncostatin M receptor (OSMRbeta). OSMRbeta is expressed in a subset of small-sized nociceptive neurons of adult dorsal root ganglia (DRGs).IL-31 and OSM may thus have redundant functions in the development of OSMRbeta-expressing neurons.

Molecular Weight: 58.2 kDa. Due to glycosylation, the protein migrates to 90-116 kDa based on Tris-Bis PAGE result.