LIFR Protein (AA 45-833) (His tag)

Catalog No. ABIN7275152

Product Details

Target: LIFR (Leukemia Inhibitory Factor Receptor alpha (LIFR))

Protein Type: Recombinant

Protein Characteristics: AA 45-833

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This LIFR protein is labelled with His tag.

Purpose: Human LIF R/CD118 Protein

Sequence: Gln45-Ser833

Characteristics: Recombinant Human LIF R/CD118 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln45-Ser833.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human LIF at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human LIF R, His Tag with the EC50 of 0.75μg/ml determined by ELISA. The affinity constant of 0.662 μM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: LIFR (Leukemia Inhibitory Factor Receptor alpha (LIFR))

Alternative Name: LIF R (LIFR Products)

Background: The leukemia inhibitory factor receptor (LIF-R) subunit is a component of cell-surface receptor complexes for the multifunctional cytokines, LIF, cardiotrophin-1, ciliary neurotrophic factor, and human oncostatin M. The structure of the human LIF-R gene is similar to that of the mouse gene. The transmembrane receptor is encoded by 19 exons.

Molecular Weight: 90.5 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Tris-Bis PAGE result.

Pathways: JAK-STAT Signaling, Growth Factor Binding